84 PARASITES BELONGING TO THE GENUS GLOMERELLA. 



GRAPE TO APPLE. 



In four different experiments in which apples were inoculated by 

 puncture with conidia from cultures from grapes or direct from the 

 host typical bitter-rot followed and acervuli developed. 



On August 19 eight Smokehouse apples were inoculated with 

 conidia from rotten grapes from another source. This strain of the 

 fungus appeared to be weak or not adapted to development on apples. 

 Rot developed on only two of the inoculated fruits. The spots 

 were small and increased very slowly and no acervuli were produced. 

 Cultures were made from these spots. 



GRAPE TO APPLE TO AGAR TO APPLE. 



On August 24 six Smokehouse apples were inoculated by puncture 

 with conidia from the culture made from apples inoculated from 

 grape as mentioned above. Decay developed very slowly about 

 the point of inoculation but a little faster than in generation 1. 

 Finally some acervuli were produced on all but one of the apples. 



On September 18 the above experiment was repeated with six 

 Smokehouse apples. The development of rot this time was some- 

 what faster than before and acervuli developed on all. 



GRAPE TO AGAR TO APPLE TO AGAR TO APPLE TO APPLE. 



Four apples were inoculated by puncture with conidia from an 

 apple inoculated with the third generation of the fungus from the 

 grape. Rot developed about as rapidly at the point of inoculation 

 on these fruits as is usual in transfers directly from apple to apple 

 and at the end of 8 days acervuli were found. 



GRAPE TO AGAR TO APPLE TO GRAPE. 



On September 23 twelve ripe berries were inoculated by puncture with 

 conidia from an apple. Only four developed the usual rot followed 

 by acervuli. The others softened somewhat but no acervuli formed. 



GRAPE TO AGAR TO GRAPE. 



On September 23 twelve ripe berries were inoculated by puncture 

 with conidia from a culture from a grape. At the end of two weeks 

 five were rotten and acervuli had formed. Seven of these even- 

 tually decayed and showed acervuli. 



GRAPE TO AGAR TO APPLE TO TOMATO. 



On September 29 four green tomatoes were inoculated by punc- 

 ture with conidia from a culture from a grape. Slight decay followed 

 in only one case, but perhaps not from the inoculation. No acervuli 

 were ever produced. 



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