INOCULATION EXPERIMENTS. 85 



GRAPE TO PUMPKIN. 



A pumpkin was inoculated by puncture with conidia from a cul- 

 ture from a grape. Rot developed rapidly and acervuli formed. 



GRAPE TO HUBBARD SQUASH. 



A nearly mature squash was inoculated by puncture with conidia 

 from a culture from a grape. Rot developed very slowly at one 

 point of inoculation only. A few acervuli were found at the end 

 of a month. 



GRAPE TO WATERMELON. 



A nearly mature watermelon was inoculated with conidia by 

 puncture from a culture from a grape. Rot developed rapidly at 

 the point of inoculation and numerous acervuli formed. 



GUAVA TO APPLE. 



Two apples were inoculated by puncture with conidia from a 

 culture from leaves of guava. Rot developed at the points of 

 inoculation about as rapidly as in the case of transfers from apple 

 to apple. Acervuli were present at the end of a week. No setae 

 were found. 



GUAVA TO BEAN. 



Six young pods of a wax bean from the greenhouse were inocu- 

 lated by surface application of conidia from guava. No signs of 

 infection ever followed. 



GUAVA TO COTTON. 



Three flowers of a cotton plant from the greenhouse were inocu- 

 lated by applying conidia from guava to the surface of the pistil. 

 No signs of infection were found at the end of two weeks, but two 

 of the small bolls which developed from the inoculated flowers 

 developed acervuli with setse when placed in a moist chamber. 



GUAVA TO PUMPKIN. 



A pumpkin was inoculated by puncture with conidia from a 

 culture from guava leaves. Rot developed rapidly and acervuli 

 were produced in abundance. 



GUAVA TO HUBBARD SQUASH. 



A squash was inoculated by puncture with conidia from a culture 

 from guava leaves. No rot appeared and no acervuli developed. 



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