160 PARASITES OF GIPSY AND BROWN-TAIL MOTHS. 
essential to success is very small. The most successful shipments 
ever received were carefully packed in shghtly dampened sphagnum 
moss, so arranged that the individual pupe rarely touched each other. 
One or two successful importations thus received in 1908 were used 
as the basis for future instructions to collectors, and in every instance 
in which the directions were carefully followed in 1909 the results 
were equally good. In 1910 additional material apparently packed 
with the same care was received from the same source and via the 
same route. For no apparent reason whatever it was worthless 
when received. . 
In consequence of this another method which has occasionally been 
followed, will be recommended for shipments in 1911. This has 
been employed successfully upon various occasions, although without 
anything like uniform success, and is in effect the same as that used 
for the shipment of full-fed and pupating caterpillars and the same 
precautions must be used. It will probably be better to place a 
somewhat larger quantity of foliage in the box to prevent the pup 
from being thrown about too much in transit. 
So very few shipments of gipsy-moth pupex have been received 
at the laboratory as to have rendered unnecessary any special devices 
for their handling after receipt. Each year a few of the lots of cater- 
pillars have contained a few individuals which were collected as pre- 
pup or as pupe, and from such, an occasional parasite of one or 
another of the species peculiar to the pupz has been reared. These 
have been so few, however, as to be of entirely inconsequential value, 
except from a technical standpoint. . 
The actual shipments of pupe collected as such have been handled 
exactly as though they consisted of active caterpillars which pupate 
en route, with the one difference that the pup received have usually 
been inclosed in darkened cages with tubes attached, in order more 
easily to remove the parasites as they issued. 
In 1908 pupz were received in satisfactory condition for the first 
time since the preliminary shipments were made in 1905, and it was 
not until then that the host relations of several of the parasites, 
notably Chalcis spp. and Monodontomerus xreus, were finally deter- 
mined. These lots were studied with the greatest care, each indi- 
vidual pupa being opened on receipt and for the most part isolated 
in a small vial, in order that it might be dissected after the contained 
parasite had issued. 
BROWN-TAIL MOTH EGG MASSES. 
No difficulty has ever been experienced in the importation of the 
ego-masses of the brown-tail moth (Pl. VII), except that when cold 
storage is not used a portion of the parasites are apt to hatch, 
and either escape or die enroute. Wooden boxes of various sizes and 
