PHYSIOLOGICAL ACTION OF VENOM OF BLACK SNAKE. 183 
Experiment I1I.—Dog, weight 14:4 kilogrammes. 
11 am., 0:15 gramme morphia acetate. 
12°10 P. m., 000025 gramme venom per kilo of body weight in 
5 cc. 9% NaCl solution slowly injected into 
jugular vein. 
20 ',, ‘00035 gramme venom per kilo. injected into jugular. 
215 ,, sample of blood withdrawn, (A 
2:30 ,, -00035 gramme venom per kilo. injected into jugular. 
30 ,, sample of blood withdrawn. (B) 
310 ,, 10cc.1% solution of CaCl, in 9% NaCl solution, 
injected into jugular. 
3°30 ,, bled todeath. (0) 
All the samples of blood were centrifugalised. The plasmas 
A, B, C clotted under the following circumstances :— 
A—Plasma clotted spontaneously in 4 hours 14 minutes 
Plasma diluted with water, in 2 hours 30 minutes - 
Plasma + CaCl, in 4 hours. 
B and O—Plasma did not clot spontaneously, nor on dilution. 
Plasma + CaCl, no clot. 
Plasma + CaCl, and 1 ce. of nucleo-albumen solu- 
tion (from testis)—precipitate, which next 
morning was partially converted into fibrin. 
Plasma acidified with acetic, hydrochloric, sulphuric 
and phosphoric acids until turbidity was pro- 
duced, clotted in less than fifteen minutes. 
Plasma + equal volume saturated solution NaCl, clotted in 
oe ten minutes. 
Plasma + equal volume saturated solution MgsSO,, clotted in 
twenty-two minutes. 
As in those cases where the negative phase is well pronounced, 
‘alcium is ineffectual in producing coagulation, both in intra- and 
extra-vascular plasma, it cannot well be maintained, as Pekelharing, 
Supposes to be the case with peptone blood, that this disappearance 
