190 Cc. J. MARTIN. 
leucocytes have no such effect, and he ascribed the consequences 
of their injection to the fluid and not to the cells. As nucleo 
albumens are set free by the disintegration of cells, Wooldridge — 
would by his washings separate the products of disintegration, © 
whereas Groth and Kriiger would inject them. 
There is then abundant evidence that the disintegration of both - 
kinds of blood cells outside the body sets free nucleo-albumens, 
which if introduced into the circulation may give rise to intra 
vascular clotting ; and also that some bodies which when injected 
into the blood stream, cause destruction of the red cells, also 
occasion thrombosis. I am therefore of opinion that the effect of 
venom on plasma is a secondary one, and due to the presence of 
nucleo-albumens in the circulation. This opinion is supported by 
the fact that in two cases, after the injection of a comparatively 
large dose of venom, in which the shed blood did not clot spon 
taneously at all, I was able to separate from the plasma, a small 
quantity of nucleo-albumen. 
Five per cent. acetic acid was carefully added to the plasm 
until the precipitate, which was at first produced, almost entirely 
disappeared. This precipitate by acidulation with acetic acid ot 
other weak acids has generally been considered to be fibrinoge 
Lilienfeld maintains however, that Hammarsten’s fibrinoge? | : 
by this means split up into two bodies : (1) “Thrombosin,” whiet 
invariably clots in the presence of calcium, (2) @ proteid, wi 
he regards as an albumose, and which itself, hinders coagulation: 
“Thrombosin” is readily dissolved by excess of the acid. N 
albumin is not dissolved by this strength of acid. 
A few more drops of the acid were then added without i 
ing what remained of the precipitate. This was then sep? 
by filtration, and thoroughly washed with water. It was 
insoluble in water or dilute saline solutions but dissolved readily 
in 1% sodium carbonate solution. Five cc. of this solution ee 
introduced into the jugular vein of a rabbit, and in jet ae . 
* duces 
1 “« Zeitschr. £. Physiol. Chem.,” Bd. ¥x: 
