SP te ee Ce Poe ee Cae eee ae eee 
PHYSIOLOGICAL ACTION OF VENOM OF BLACK SNAKE. 203 
upon the organism by sojourn in the serums, was ascertained 
from time to time by withdrawing a wire loopful and mixing this 
with 10 cc. of nutrient gelatine and making a plate cultivation 
of the mixture. An indication of the extent to which the growth 
of the bacilli had progressed in the serum tubes was obtained by 
counting the number of colonies appearing in the plate cultivations 
after definite intervals. 2 
The following notes represent some few of the results of these 
experiments in tabular form, and clearly show that whereas the 
growth of the organism was as usual hindered by the normal 
serum, on the other hand the serum from the poisoned animal 
formed an excellent culture medium. In this particular case the 
damaging influence of the normal serum was less than is usually 
the case. 
The tables represent. the number of colonies of the organism 
which developed in the plate cultures after various intervals of 
time,! 
I.—After Five Minutes Sojourn in Serum. 
Number of Colonies after 
18 hours. | 21 hours.| 48 hours. 
Normal serum ....|_——-22 | 123 
Venom serum _...|. 39 | 464 | 560 ! 
II.—After One Hour’s Sojourn in Serum. 
Number of Colonies after 
17 hours. | 20 snes 42 hours. 
[i 
| 
sf 
x 
Normal serum 12 672 ge 
. _ Venom serum _ 987 | 2000 | countless 
ee TII.—After Two Hours’ Sojourn in Serum. 
: Number of Colonies after 
d 16 hours. 19 hours,| 41 hours. 
Normal serum... 5 | 560 
Venom serum 1450 | 2416 | countless | 
: Sa a ent we teeretientinie ale nee Sa 
1 
For the of greater portion of the troublesome details 
of the the performane 
Mr. R. — I ses 1 indebted to my friends Dr. F. — and 
‘ 
