252 C. J. MARTIN. 
Experiment VI.—Frog (male), weight 20 grammes, prepared as 
usual, 
4°30 p.m., 5 ce. of a 2% solution of venom previously heated to 
85° C. injected into dorsal lymph sac. 3 
4:45 ,, respiratory movements ceased ; recovers with difficulty 
when placed on back. 
4:50 ,, all reflexes absent ; sciatics prepared for stimulation. 
Maximum distance between coils when gastrocnemius 
-ontracted. 
Electrodes on | Electrodes on 
sciatic nerve. dorsal cord. 
| Ligatured limb... 75 cm. 37 cm, 
Unligatured limb 79 cm. 34 cm. 
Experiment VII.—Frog (female), weight 18-5 grammes, prepared 
as usual. 
4:30 p.m., ‘5 cc. of a 2% solution of venom previously heated 
to 85° C. injected into dorsal lymph sac. a 
4:40 ,, respiration shallow; reflexes dull; recovers with — 
difficulty when laid on back. 
5°35 ,, no reflex response ; sciatics dissected out. 
Maximum distance between coils when gastrocnemius 
| Electrodes on | Electrodes on 
sciatic nerve. | dorsal cord. ; 
| Ligatured limb... 61 cm. 32 cm. : 
| Unligatured limb} 58 cm. 33 cm. ‘ 
In nearly the whole of these experiments the contraction of the 
gastrocnemius was induced by a less stimulus in the poisoned is 
in the unpoisoned limb. Had the poison exerted any acs 
analagous to that produced by curari, the opposite result W si 
have been observed. The diminished sensitiveness of the muscles 
in the ligatured limb is explained by the fact that all cireulatio® L 
had been cut off since the application of the ligature. The long? 
the time which had elapsed since the limb was tied, the gre 
was the difference in sensitiveness between the two sides. 
