80 



Bokorny 1 ), and many other authors have investigated their poisonous 

 effects upon the Protozoa, Coelenterata, Echinodermata, Vermes, Arthro- 

 poda, Mollusca, and VeHebrata, while Maecet 2 ), Knop 3 ), Detmer 4 ), 

 Marcacci 5 ), Bokorny 6 ), Varigny 7 ), Loew 8 ), Lutz 9 ), Planchon 10 ), 

 Clautriau 11 ), and others have made the same study on the cryptogams 

 and phanerogams. Concerning fungi, Lutz and Planchon may be here 

 especially mentioned : the former investigated the influences of chlorides 

 of quinine, cocaine, morphine, piperidine, sulphate of atropine, etc. upon 

 Penicillium and Aspergillus, and the latter those of quinine sulphate and 

 cocaine chloride upon Gladosporium, Alternaria, and Dematium. 

 For culture I used Richards' solution 12 ): 



Distilled water - . . 100 ccm 



Cane-sugar 5 g 



Ammonium nitrate 1 „ 



Monopotassium phosphate 0.5 „ 



Magnesium sulphate 0.25 „ 



Ferric sulphate trace ; 



to which I added each alkaloid, so as to obtain the concentrations of 

 0.2o/ O} Q.5 o/ , \o/ 0) 1.5%, 2o/ 0) etc. 



Experiments. 



L Tropinc-group ' 3 ). — 



(a) Cocaine chloride. Penicillium glaucum and Aspergillus niger 

 could bear more than 3 o/ of this salt. In the cultures of higher con- 

 centration the conidia formation was retarded and reduced. Botrytis 

 cinerea grew well in the solutions of 1 % and even stronger concen- 



x ) Bokorny. Pfliig. Arch. 1896. Bd. LXIV. 



a ) Maecet. Ann. de chim. et phys. 1825. T. 29. 



3 ) Knop und Wolf. Landwirthsch. Versuclisstationen. 1865. Bd. VII. 



*) Detmek. Landwirthsch. Jahrb. 1881. Bd. X, 



5 ) Marcacci. Ann. di chim. e di farmacol. 1887. 



°) Bokorny. be. cit. and Jahrb. f. wiss. Bot. 1888. Bd. XIX. 



7 ) Varigny. Revue generate de Bot. 1892. T. 4. 



8 ) Loew. Ein naturliches System der Giftwirkungen. Munchen 1893. 

 °) Lutz. Ann. d. Sc. nat. Bot. 1898. Ser. VIII, T. 7. 



*°) Planchon. Ann. d. Sc. nat. Bot. 1900. Ser. VIII, T. 11. 

 1 x ) Clautriau. Nature et signification des alcaloides veg£taux. Bruxelles 1900. 

 12 )H. M. Richards. Die Beeinflussung des Wachsthums einiger Pilze durch 

 chemische Reize. Pringsh. Jahrb. f. wiss. Bot. 1897. Bd. XXX. p. 667. 



13 ) V. v. Richter. Chemie der Kohlenstoffverbindungen. Bonn 1898. Bd. II. p. 610 



