150 — 



We are now carrying out the examination method 1 ) in a somewhat 

 modified form, which enables us also to determine the constants, and in 

 particular the original ester-content of the oil after it has been extracted 

 and freed from the added glyceryl ester. To do this we shake up 10 cc. 

 oil with 20 cc. of 5°/o alcohol without the addition of light petroleum. 

 When the layers have cleared, (a process requiring more time than for- 

 merly with bergamot oil, but taking place quickly enough with oils of 

 lavender and petitgrain), 10 cc. of the filtrate, which should be as clear 

 as possible, is neutralised after adding a few drops of phenol phthalein 

 solution, and saponified for one hour with 5 cc. seminormal potash 

 liquor. With pure oils the alkali used up amounted up to 0,1 cc. of semi- 

 normal liquor. 



The proof that the three lavender oils in question were adulterated 

 was afforded by the figures showing the quantity of seminormal liquor 

 used up, which were as follow: — 



Sample No. 1 = 1,16 cc, No. 2 = 0,79 cc, No. 3 = 4,77 cc. 



The constants of these oils before and after extracting with 5°/o alcohol 

 were as follow: — 



Nr. 1 

 before after 



Nr. 2 

 before after 



Nr. 3 

 before after 



dis 



«D 



"D20 



sol. in 70°/o ale. 



acid v 



ester v. V 2 nour 

 ester v. 1 hour 

 linalyl acetate . 



sap. v 



acid v. II. . . . 

 difference. . . . 



0,8943 



— 6° 2' 



1,46169 



3 Vol. a.m. 



0,8 



116,0 



116,9 



40,9 °/o 



117,7 



117,4 



0,3 



0,8892 



1,46278 

 0,6 



104,1 



36,4 °/o 



0,8908 



1,46238 



0,5 



108,9 

 38,1% 



0,9128 



— 3° 56' 



1,46169 



2 Vol. a. m. 



0,9 



110,8 



111,4 



39°/o 



112,3 



111,1 



1,2 



0,8990 



— 4° 6' 



1,46248 



2 Vol. a. m. 



0,5 



81,0 

 28,3°/* 



0,8923 



— 5° 4' 



1,46120 



2,8 Vol. a.m. 



0,5 



112,2 



112,2 



39,3 °/o 



112,7 



111,5 



1,2 



The values of samples Nos. 1 and 2 were quite normal and the ad- 

 ulteration which had been practised with them could only be detected 

 by testing for glyceryl ester, but sample No. 3 was suspect from the 

 beginning on account of its high specific gravity. In this case also 

 however, the adulterant which had been employed could only be detected 

 by the special test for glyceryl ester. 



The quantity of sample No. 1 at our disposal amounted to a few cubic 

 centimeters only, and it was therefore impossible to isolate the foreign 

 ester directly, but of Nos. 2 and 3 we had considerable quantities in our 

 possession, and we were able to remove the added ester from them by 

 taking 1 kilogram of each oil and shaking it up three times with 1 kilogram 

 of 5°/o alcohol. The alcoholic solution, after being passed through a 



!) Report October 1910, 61. 



