| NOTES ON SCIENTIFIC RESEARCH. 79 
Determination in plants of some glucosides hydrolysable by emulsin. 
E. Bourquelot and his collaborators have again published some papers on the action 
of the emulsin enzymes and the occurrence in plants of glucosides hydrolysable by 
emulsin’). 
Through the action of emulsin on anisyl alcohol dissolved in acetone, Bourquelot 
and Ludwig”) obtained §-anisyl glucoside, an odourless mass, which, in anhydrous 
state, melts at 137 to 138° on Maquenne’s block. This glucoside possibly occurs in 
vanilla beans. 
Together with Bridel, Bourquelot*) describes the determination of glucosides, 
hydrolysable by means of emulsin, in the orchids Aceras anthropophora, R. Br.; Loro- 
glossum hircinum, Rich.; Orchis purpurea, Huds.; O. Morio, L.; O. maculata, L.; O. lati- 
folia, L. and O. conopsea, L. As to the nature of these glucosides, the authors have 
nothing further to communicate as yet. 
Finally, Bourquelot and Bridel*) report on the fermentative equilibrium and the 
specific action of enzymes, as regards their synthesizing properties. 
Occurrence of mustard oils in some Cruciferae. 
In order to prove the presence of mustard oils in some Cruciferae, J. ]. Blanksma’) 
prepared combinations of these bodies with ammonia and phenylhydrazine. He distilled 
with steam the minutely powdered herb of Cochlearia officinalis, L., which had previously 
been treated with water, thus obtaining from flowers 0,062 p.c., from leaves 0,009 p.c., 
from the stalks 0,004 p.c., and from the whole plant 0,0305 p.c. of d-sec.-butyl mustard 
oil. With phenylhydrazine in hot alcohol, it forms phenyl-d-sec.-butyl-thiosemicarbazide, 
melting at 135°; with methylphenylhydrazine, 1-methylphenyl-4-d-sec.-butylthiosemi- 
carbazide, melting at 112°. 
From the herb of Cochlearia danica, L. only 0,006 to 0,007 p.c. of volatile oil was 
obtained, which consisted in the main of d-sec.-butyl mustard oil. Lady’s smock 
(Cardamine pratensis, L.) yielded on distillation 0,00135 p.c. of oil, which proved to be 
d-butyl mustard oil. The yield from Cardamine hirsuta, L. was still smaller; this 
mustard oil could not be identified for certain, however. 
Shepherd’s purse (Capsella Bursa pastoris, Mnch.) did not, on steam distillation, yield 
any mustard oil, but a mercaptane. 
Tropaeolum seeds (Tropaelum majus) contain benzyl mustard oil, which with phenyl- 
hydrazine yields 1-phenyl-4-benzylthiosemicarbazide (m. p. 158°). 
The distillates of Lepidiwm campestre, Nasturtiwm amphibiwm and Draba verna strongly 
hindered the development of yeast. 
Allyl mustard oil from mustard seeds gave with phenylhydrazine phenylallylthio- 
semicarbazide, melting at 118°; with methylphenylhydrazine, the corresponding methyl 
compound (m. p. 90°). 
Anemonin in Ranunculus japonicus. 
In some Ranunculaceae, f.i1. anemoneae, there occurs an acrid substance, anemonin, 
Cio0H;0.1, which volatilizes with steam and which was investigated many years ago by 
H. Beckurts*®) and later on by H. Meyer’). Meyer established a formula for anemonin, 
1) Comp. Report April 1914, 125. — 2) Journ. de Pharm. et Chim. VII. 9 (1914), 542. — 8) Ibidem VII. 
10 (1914), 14. — +4) Ibidem VII. 9 (1914), 230, 603. — 5) Pharm. Weekbl. 51 (1914), 1383; Apotheker Ztg. 29 
(1914), 950; Chem. Zentralbl. 1915, 1. 262, — %) Arch. der Pharm. 280 (1892), 182. — 7) Monatsh. f. Chem. 
17 (1896), 283; 20 (1899), 634; Chem. Zentralbl. 1896, Il. 303; 1899, II. 780. 
