PRESERVATION OF SPECIMENS IN PLASTICS 51 



saturated aqueous solution and in combination with another preserving 

 agent such as acetic, chromic, or picric acid. 



6. Formol-alcohol. — This frequently used preserving fluid can be 

 prepared by adding 10 parts of formalin to 90 parts of 50-percent 

 alcohol. Where desired 70-percent alcohol may be used instead of the 

 50-percent. 



7. Alcohol-acetic acid. — (Carnoy's Fluid 3:1) Mix 95-percent alcohol, 

 3 parts, with glacial acetic acid, 1 part. The mixture may be diluted 

 with water as required for various types of specimens. 



8. Formalin-alcohol-acetic acid. — One mixture (Dietrich's or Kahle's 

 Fluid) comprises: 95-percent alcohol, 30 cc; formalin, 10 or 12 cc; 

 glacial acetic acid, 2 cc; distilled water, 60 cc. A second mixture 

 (Lavodowsky's Fluid) comprises: 95-percent alcohol, 50 cc; formalin, 

 10 cc; glacial acetic acid, 2 cc; and distilled water, 40 cc. A third 

 mixture (Wells' Fluid) comprises: 50-percent alcohol, 100 cc; formalin, 

 6.5 cc; and glacial acetic acid, 2.5 cc. 



9. Chrome-acetic acid. — According to Wells, two mixtures are used. 

 One comprises 1-percent chromic acid solution, 100 cc, and glacial 

 acetic acid, 5 cc. The other comprises 1-percent chromic acid solution, 

 100 cc, and glacial acetic acid, 10 cc. 



10. Chrome-acetic acid-formalin. — Mix 16 parts of 1-percent aqueous 

 solution of chromic acid with 1 part of glacial acetic acid. Just before 

 using, add one volume of formalin to two volumes of this solution. 



11. Alcohol-chloroform-acetic acid. — (Carnoy's Fluid 6-3-1). — Mix 

 6 parts of 95-percent alcohol, 3 parts of chloroform, and 1 part of 

 glacial acetic acid. 



12. Carnoy-LeBrun Fluid. — Saturate a mixture of equal parts of 

 absolute alcohol, glacial acetic acid, and chloroform with mercuric 

 chloride. 



13. Kleinenberg's Fluid. — Add 98 volumes of a saturated aqueous 

 solution of picric acid and 2 volumes of concentrated sulfuric acid to 

 200 volumes of water. 



14. Bouin's Fluid. — Mix 75 cc of a saturated aqueous solution of 

 picric acid with 25 cc of formalin and 4 cc. of glacial acetic acid. 



15. Rabl's Fluid. — Add 1 volume of a saturated aqueous solution of 

 picric acid and 1 volume of a saturated aqueous solution of mercuric 

 chloride to 2 volumes of distilled water. 



16. Rath's Fluid. — Mix 1 volume of saturated aqueous solution of 

 picric acid, 1 volume of hot saturated aqueous solution of mercuric 

 chloride, and from one-half to 1 volume of glacial acetic acid. 



17. Van Leeuwen's Fluid. — Mix 24 cc. of 1-percent solution of picric 

 acid in absolute alcohol, 4 cc. of chloroform, and 4 cc of formalin. 

 Before using, add 2 cc. of glacial acetic acid. 



18. Schaudinn's Fluid. — Mix 2 parts of a saturated aqueous solution 

 of mercuric chloride with 1 part of 95-percent alcohol. 



19. Worcester's Fluid. — Prepare a fresh saturated solution of mer- 

 curic chloride in 10-percent formalin. If desired, 9 parts of this solution 

 may be mixed with 1 part of glacial acetic acid. 



20. Zenker's Fluid. — Add 2.5 gm. potassium dichromate, 5 gm. mer- 

 curic chloride, 1 gm. sodium sulfate, and 5 cc. glacial acetic acid to 

 100 cc. water. The acetic acid should be added just before use. 



In addition to treating specimen material with preserving fluids or 

 fixatives, it may be found desirable in some cases to increase their 



