THE MOSQUITOES OF THE SOUTHEASTERN STATES 9 
Uranotaenia larvae and certain species of Culex, especially C. erra- 
ticus, are taken frequently with anophelines. Many other mosquito 
larvae, however, particularly those of Aedes and Psorophora, are more 
active and usually drop to the bottom of the pool as soon as disturbed. 
A quick plunge of the dipper is required to intercept these larvae, or 
they may be collected by sweeping through the water with a cloth col- 
lecting net or a fine-meshed wire strainer. Other kinds of mosquitoes, 
such as Mansonia, Wyeomyia, the tree-hole breeders, etc., require a 
special technique depending upon the character of the breeding place. 
i; iS : = 
E ‘ eS | 
& SSeS SeeRaS Sees SSS Saks 8 SSS SSIS SS SESS ec ON 
Figure 5.—Large-mouthed pipettes for collecting larvae and pupae, and chloro- 
form killing tubes for collecting adults. The first of the two tubes is equipped 
with a paper funnel. 
As soon as a collection is made, the jar should be numbered and a 
record kept of the locality, date, and conditions under which the 
larvae were found. 
The larvae and pupae may be kept alive for rearing, or the large 
larvae (fourth instars) may be preserved for identification in 70- to 
80-percent alcohol or 10-percent commercial formalin. About 1 per- 
cent of glycerin should be added if the vials are to be stored. Speci- 
mens retain their form best if killed in hot water (not over 150°F.). 
422447—42 
