protein amino acids and ammonia accounted 

 for 3.23 percent of total feces nitrogen. 



139. Moore, R. F., Jr., and Taft, H. M. 

 Effect of DDT and toxaphene alone and in 

 combination on succinic dehydrogenase ac- 

 tivity in homogenates of the boll weevil. Jour. 

 Econ. Ent. 57: 772-773. 1964. 



The phenazine methosulfate method used in 

 vertebrate-tissue studies was used. It measures 

 oxygen uptake as a function of enzyme activity. 

 No striking effects of DDT, toxaphene, or a 

 combination of these two insecticides on the 

 activity of succinic dehydrogenase were shown. 

 It is suggested that the effects of DDT on the 

 activity of the enzyme in other insects, as re- 

 ported by some investigators, may have been 

 due to the less specific method employed. 



140. Moore, R. F., Jr., and Taft, H. M. 

 Some in vitro effects of dinitrocresol and mag- 

 nesium chloride on ATPase in the boll weevil. 

 Ent. Soc. Amer. Ann. 57: 28-31. 1964. 



In a study of the activity of ATPase in ho- 

 mogenates of the boll weevil sufficient magne- 

 sium occurred to activate the enzyme. Additional 

 magnesium did not increase activity of the 

 enzyme. A higher level of ATPase occurred in 

 a laboratory-reared than in a field-collected 

 population. Dinitrocresol added to homogenates 

 containing no exogenous magnesium ions in- 

 creased ATPase activity but depressed it when 

 5xl0 3 M magnesium chloride was present. 



141. Moore, R. F., Jr., Taft, H. M., and 

 Whisnant, F. F. Effect on boll weevil prog- 

 eny of cholesterol added to the adult diet as a 

 powder or an ether solution. Jour. Econ. Ent. 

 57: 1005. 1964. 



Adults fed on an artificial diet to which cho- 

 lesterol was added as an ether solution pro- 

 duced more viable eggs and larger progeny 

 than those fed on a diet to which cholesterol 

 was added as a powder. 



142. Neff, D. L., and Vanderzant, E. S. 

 Methods of evaluating the chemotropic re- 

 sponse of boll weevils to extracts of the cotton 

 plant and various other substances. Jour. 

 Econ. Ent. 56: 761-766. 1963. 



Two methods are described. In one, solutions 

 of pure chemicals or extracts of the cotton plant 

 were applied to a feeding dish containing diet. 

 In the other, plant parts were placed in a small 

 container covered with a paraffin film to pre- 

 vent contact of the test material by the insect. 

 The behavior of the boll weevil in both assay 

 methods is described, and the applications of 

 the methods are discussed. 



A solvent extraction of cotton-plant parts 

 and the chromatography of the extract are de- 

 scribed. By the use of the diet method, several 

 fractions attractive to the boll weevil were 

 found. 



143. Nettles, W. C, Jr., and Betz, N. L. 

 Glycogen in the boll weevil with respect to dia- 



pause, age, and diet. Ent. Soc. Amer. Ann. 

 58: 721-726. 1965. 



On a dry-weight basis, the glycogen content 

 of the boll weevil is maximum (11 percent) in 

 the eggs. It is about 1.5 percent through most 

 of the larval period, but it rises to 6 percent 

 late in the last larval instar. It declines rapidly 

 in the first half of the pupal period but less 

 rapidly in the second half, and the minimum 

 titer (99 percent less than in the late last 

 larval instar) is found in the 1-day-old adult. 

 Adults, whether fed on bolls or squares, have 

 their highest glycogen content at 6 to 15 days 

 of age, but the titer is several times higher in 

 boll-fed than in square-fed weevils. This phe- 

 nomenon and the higher triglyceride content of 

 boll-fed individuals probably result from the 

 five to eight times greater sugar content (prin- 

 cipally glucose and fructose) in bolls as com- 

 pared with squares. In boll-fed adults the 

 glycogen titer differs little or not at all between 

 diapausing and reproducing weevils, but in 

 square-fed weevils it is higher in those that are 

 diapausing, though still below the level found 

 in boll-fed, reproducing weevils. The glycogen 

 titer of whole weevils decreases during storage 

 at -20°C, but this loss was prevented by storing 

 the weevils in ethanol at -20°. 



144. Oliver, A. D., and Sloane, L. W. Ef- 

 fects of various water volumes on the effective- 

 ness of methyl parathion-DDT in controlling 

 the cotton boll weevil, Anthonomus grandis. 

 Jour. Econ. Ent. 57 : 292. 1964. 



There were no differences in boll weevil con- 

 trol or yield of cotton when treatment was 

 made with constant rates of methyl parathion 

 and DDT in 2,5, or 8 gallons of total spray per 

 acre. 



145. Parencia. C. R., Jr., Davis, J. W., and 

 Cowan, C. B., Jr. Studies on the ability of 

 overwintered boll weevils to find fruiting cotton 

 plants. Jour. Econ. Ent. 57: 162. 1964. 



Experiments during 1960, 1961, and 1962 in 

 central Texas showed that overwintered boll 

 weevils emerging from hibernation sites found 

 groups of five and of 20 fruiting plants more 

 readily than they found single plants. Some 

 weevils were collected by this method near the 

 hibernation sites in early spring before field- 

 grown plants were available. However, the 

 method was not of practical value in reducing 

 overwintered populations. 



146. Parish, J. C, and Arthur, B. W. 

 Mammalian and insect metabolism of the che- 

 mosterilant thiotepa. Jour. Econ. Ent. 58: 

 976-979. 1965. 



Thiotepa was synthesized with a P 32 label, 

 and its metabolic fate was studied in white 

 rats and four species of insects, including the 

 boll weevil. 



Maximum absorption after topical applica- 

 tion occurred by 4 hours after treatment in all 

 insects except the boll weevil. 



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