10 MISC. PUBLICATION 336, U. S. DEPT. OF AGRICULTURE 



As soon as a collection is made, the jar should be numbered and 

 a record kept of the locality, date, and conditions under which the 

 larvae were found. 



The larvae and pupae may be kept alive for rearing, or the large 

 (fourth-instar) larvae may be preserved for identification in 70- to 

 80-percent alcohol or 10-percent commercial formalin. About 1 per- 



1 



Figure 5. — Large-mouthed pipettes for collecting larvae and pupae, and chloro- 

 form killing tubes for collecting adults. The first of the two tubes is equipped 

 with a paper funnel. 



cent of glycerin should be added if the vials are to be stored. Speci- 

 mens retain their form best if killed in hot water (not over 150°F.). 

 Permanent slide mounts of the larvae (or of the male genitalia) 

 are conveniently made with Berlese's chloral-gum solution or one 

 of its modifications. Gater's (45) slight modification of this formula 

 is as follows : 



Gum arabic (picked) grams 8 



Distilled water milliliters — 10 



Chloral hydrate grams — 74 



Glucose sirup (10 g of glucose in 10 ml of water) 



milliliters— 5 



Glacial acetic acid do 3 



