126 TREATISE ON THE 
testifying their affection and regard; one 
after another presented her proboscis with 
food, and at every step of her progress, a 
circle was formed around her by her admi- 
ring subjects. The other circumstance allu- 
ded to, which varies from the experience of | 
Huber, respects the vigilance of the workers 
in such cases, and the sound emitted by the 
queens. He says, that the workers form no 
guard around the cells of artificial queens, 
and that these last are perfectly mute; and 
the naturalist makes some remarks by way 
of accounting for it. The above experiment 
is completely in contradiction to this. The 
cell of the younger queens was most strictly 
guarded, and both emitted the sounds allu- 
ded to, perhaps once every minute, for several 
hours together. 'T’o these experiments I have 
only to add farther, that, as already stated, I 
have very frequently repeated the same ope- 
ration and always with success; and that. 
in the summer of 1841, 1] removed the reign- 
ing queen of the same Observatory hive three 
times successively, supposing each queen to 
