18 Department Circular 284, U. S. Dept. of Agriculture 



was apparently still incomplete, probably because a trapped air 

 bubble had prevented sufficient solution from passing through the 

 opening. 



Table 7. — Cultural results of various tests icith samples of comb treated for 48 

 hours in commercial alcohol-formalin solution 





> o 



s » 3 

 8S.S 



£§ = 



Jo ® 



■§|i 



1-a.s 



Open cells 



First comb in series contain- 

 ing open cells showing 

 growth of B. larvae in cul- 

 tures 



Sealed cells 



a M .S 



Us 





■a 



o 



CO 



<D 



-a 

 8*8 



o S 

 o 



a 



3 



5 



P s> 



*,§ 



o 



■S«5 



o 



ll 



— i u 

 3 M 



o 



o 



a 

 to 

 a 



Is 



■si 



3 



3 

 o 



3 

 o 



ca 



CD 



13 



co T3 



a 



9 



M u 



.s§ 



o~ 



*o 



co ja 



11 



"3 M 



o 



o 



a 



bo 



a 



is 

 ■si 



3 

 o 



First comb in series cor 

 ing sealed cells she 

 growth of B. larvt 

 cultures 





i 4 

 3 

 3 

 7 

 7 

 8 

 5 

 7 

 7 



. 10 



/ io 

 1 io 



10 



10 



10 

 f 10 



do 



i 



3 

 7 



i 



5 

 7 

 7 



10 

 50 

 50 

 50 



50 



50 

 10 

 10 





 

 

 

 

 

 

 

 

 

 

 

 







'3 

 

 



4 

 3 

 3 

 7 



7 

 8 

 5 

 7 





4 



3 



3 



7 



7 



8 



5 



7 



7 



10 



40 



40 



50 



14 

 1 

 1 



i 2 

 1 

 2 



*1 



5 



3 



2 



I 10 



3 18 



4 25 







2 



2 



5 



6 



6 



4 



2 



4 



8 



30 



22 



25 



First. 













First. 







Fifth. 







Third. 



Preliminary tests 





First. 

 Third. 















7 



... ._ 



First. 





10 

 50 

 50 

 50 



50 



47 

 10 

 10 





Ninth. 



Open (uncapped) cells compared with sealed cells; 





Third. 





Do. 



Open (uncapped) cells compared with sealed cells; 



fresh solution. 

 Fresh solution; control for samples washed after 



treatment. 



Open cells, washed in water after treatment 



Cells with perforated cappings compared with 



open cells. 





First. 



















16 6 



10 1 



10 

 9 







Tenth. 













' One showed few spores germinated. 

 > Few spores germinated. 



* Three showed few spores germinated. 

 4 Eight showed few spores germinated. 



Table 8. 



-Cultural results of various tests with samples of comb treated for 2% 

 hours in commercial alcohol-formalin solution 



8-1 



o «» 



fe^a 

 i£a 



Preliminary tests 



Uncapped cells only; used solution 



Uncapped cells only; fresh solution 



Fresh solution; control for samples washed 



treatment; uncapped cells only. 



Open cells, washed in water after treatment 



Cells with perforated cappings compared with 



open cells. 



Open cells 



i 



M *» 



a e 



CO 



H 







S'V 



to 2q 











O 3 







ss 





,Q 



3 E 



a 



3 



£ 



"3 Eb 



fe 



O 



5 







50 



1 2 



50 







50 



1 1 



50 



»3 



50 



'4 



10 







10 







Bo 3 



Sealed cells 







Ofl' M 



r/l « 



q 



M 



,*i 







3 



IS| 





 8 



1 



M 



a 



5 8 «t 



-a 



o-n 



CO _ • 



is 



!Sgo 



03 



"o 



■si 



8^8 



+j MO h 



a 



3 t 

 ~ 



3 



<-> 



fj.a mxs 



3 



3 tj 

 O 



3 



ft 



a 



U 





fi 



2 



3 



First 

















Tenth... 









Sixth. . 









First 











10 

 10 



3 

 '2 



7 

 8 







o a 



8^ 



agxi 

 8 M £ 



i-.3 M 



5 



First. 



Eighth. 

 Fourth. 





Few spores germinated. J One showed few spores germinated. * Two showed few spores germinated. 



