The Sterilization of American Foulbrood Combs H 



expensive for practical use, was also tested. Only four series, each 

 of seven cells, were tried with this carrier for purposes of com- 

 parison, two of open and two of sealed cells, the proportions used 

 being 50 parts of iso-propyl alcohol, 30 parts of water, and 20 

 parts of formalin. Of all the 28 cells cultured only one sealed cell 

 was found to contain viable Bacillus larvae. This solution is there- 

 fore in about the same class as that of acetone. 



MISCELLANEOUS SOLUTIONS 



The results of experiments with these solutions are presented in 

 Table 3. The use of iodine solutions proved entirely impracticable 

 for sterilizing infected combs. In dilutions of 1 to 50,000 in water 

 and even 1 to 500, with an immersion of 48 hours, no germicidal 

 action on the spores from diseased material in either open or sealed 

 cells could be demonstrated. A solution of 1 to 20 iodine killed all 

 the spores, both in open and sealed cells, but the comb was attacked 

 to such an extent as to make it too soft for further use. The probable 

 reason for the lack of germicidal action on the part of iodine is 

 that this substance combines readily with the fatty-acid constituents 



Table 3. — Cultural results of various tests with samples of comb treated for 24 

 hours in various solutions 





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Todinp, 1-500 water 



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Tndine', 1-20 water 





Denatured alcohol (50 per cent), 93 





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c. c, hydrochloric acid, 5 c. c, 

 formalin, 2 c. c. 



Denatured alcohol, 90 c. c, hydro- 

 chloric acid, 5 c. c, formalin, 5 c. c. 



Denatured alcohol (50 per cent) , 93 



Third - . 



Do. 

 Do. 



c. c, hydrochloric acid, 5 c. c, 

 formalin, 2 c. c. 



Denatured alcohol, 90 c. c, hydro- 

 chloric acid, 5 c. c, formalin, 5 c. c. 



Acetic acid, 10 c. c, water, 70 c. c, 



First-... 



Do. 



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formalin, 20 c. c. 

 Do.... 





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Acetic acid, 5 c. c, water, 75 c. c, 





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formalin, 20 c. c. 

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20 c. c, glycerin, 2 c. c. 

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1 per cent solution of gelatine, 80 





Second. 



c. c, formalin, 20 c. c. 

 Do 





Do. 





Do. 



95 per cent alcohol, 80 c. c, forma- 

 lin, 20 c. c. 







1 One showed few spores germinated- 



Few spores germinated , 



