Cell Cultures 



(1) Kinds of cells 



(2) Cell nutrients 



(3) Virus yield 



(4) Viral changes 



(5) Interference 

 Control Measures 



(1) Virus persistence in animals, products, and 

 environment 



(2) Carrier studies 



(3) Pathology and diagnosis 



(4) Epizootiology and host range 



(5) Disinfection 

 Diagnostic Tests 



(1) Complement fixation 



(2) Neutralization 



(3) Agar gel diffusion 



(4) Fluorescent antibody 



(5) Agglutination 



RESEARCH DISCIPLINES 



The five research disciplines are biochemical 

 and biophysical, immunological, cytological, 

 microbiological, and diagnostic investigations. 

 These research disciplines are composed of 

 veterinarians, virologists, bacteriologists, patho- 



logists, chemists, physicists, and their technical 

 assistants. Working alone or as teams, the 

 scientists and their assistants are assigned to one 

 of the five research disciplines. 



Biochemical and Physical 



Scientists in biochemical and physical investi- 

 gations are concerned with problems in mole- 

 cular biology. These scientists produce milligram 

 quantities of foot-and-mouth disease virus in 

 cultures of baby hamster kidney cells and purify 

 the virus for use in biochemical and immunolo- 

 gical studies. They examine animal virus 

 particles for their size and shape by electronmic- 

 roscopy and for their chemical properties, 

 including resistance to mechanical treatment, pH 

 changes, thermal changes, and variations in ionic 

 strength. They determine the effects of enzymes 

 and chemicals as purifying agents and inacti- 

 vants; study viruses intact and broken down into 

 their protein and infectious nucleic acid subu- 

 nits; determine diffusion, electrophoretic, and 

 sedimentation rates of viruses and their sub- 

 particles. 



The scientists in this group also investigate the 

 correlations of physiochemical properties with 

 infectivity, immunogenicity, and antibody - 



PN-3658 



An electron micrograph shows foot-and-mouth disease virus particles. 



