293 
in the vom Ratu mixture (picric + osmic + acetic + platinic chloride) 
eight days. It was then washed for a short time in methyl alcohol 
followed by pyroligneous acid for forty-eight hours. Thence it was 
transferred to absolute alcohol, where it was allowed to remain for 
several days with frequent changes of al- 
cohol. Farther staining was unnecessary. Si 
The sections were cut 31/, u thick, were 
mounted in Canada balsam and gave most 
satisfactory results. The material prepared 
by the sublimate method, although in 
most cases satisfactory, sometimes showed 
shrinkage of the cell protoplasm. 
The nervous system of this Maldanid, 
which I shall describe more fully in a , | 
future article, is peculiar and interesting. O 
It seems to show a very primitive condi- ae 
tion, for it lies in the hypodermis and in 
each segment there are given off on either 
side of the body a large number of nerves 
— thirty-five or more. Along the mid- 
dorsal region of the cord extends a single 
“neurochord tube” or “giant fibre”. 
The ganglion cells differ much in 
size. Small cells are found abundantly 
throughout the length of the cord, both 5 
laterally and ventrally. Besides these | 
small cells, there are found a limited 
number of very large cells, which I shall ||| © 
call by the well-known name “giant cells”, 
although they differ much in detail from i A 
the cells which I have found described 
under that name. | 
_ 
These giant cells are the ones which O 
I propose to describe somewhat minutely. Ie 
They are arranged unsymmetrically and 
without any discoverable regularity along 
the cord; more generally, it is true, at all is : 
1 2. 
Figures 1 and 2 are diagrams of the nerve cord in the ninth and tenth segments 
respectively, to show the distribution of the giant ganglion cells and their relation to 
the “neurochord tube”. The horizontal lines mark the position of the setae. 
