535 
of newer and more satisfactory methods, further information regarding 
the peculiar globule. The ova, which are still in a most admirable 
state of preservation, were fixed in FLEMMING’s fluid or corrosive 
sublimate, and have since been kept in 70°/, alcohol, the precaution 
having been taken of changing the alcohol occasionally. I had found 
by my earlier experience that the ova, from the large amount of yolk 
which they contain, become exceedingly brittle when imbedded in 
paraffin, and they were therefore imbedded in celloidin and the sections 
were stained, either individually or on the slide by the method described 
by EYCLESHYMER, in HEIDENHAIN’sS iron-alum haematoxylin. 
From these preparations it is clear that I was entirely in error 
in identifying the structure I had seen as a polar globule, since it 
occurs at the vegetative pole of the egg and contains no nucleus. 
It is composed of yolk granules 
closely packed together (Fig. 1) 
and is undoubtedly simply a 
yolk-lobe comparable to that 
which occurs in many Gastero- 
pods. The true polar globules 
are very much smaller and en- 
tirely destitute of yolk, and are 
two or three in number, the 
third being formed by the division 
of one of the two usually found, 
as frequently happens in the 
Mollusca. 
Fig. 1. Section through egg of Fulgur showing the yolk-lobe at the vegetative 
pole. Only a few of the yolk granules are represented. 
The occurrence of such a minute yolk-lobe in an ovum so abundantly 
supplied with yolk as is that of Fulgur is very interesting. A 
yolk-lobe has been described as occurring in several Gasteropods and 
it has also been found among the Annelids. E. B. Wırson!) and 
MAD 2) have described on one of the macromeres of Chaetopterus a 
1) E. B. Wırson, Observations on the early developmental Stages of 
some po!ychaetous Annelids. Studies Biol. Labor. Johns Hopkins Univ., 
II, 1886. 
2) A. D. Mran, Some Observations on Maturation and Fecundation in 
Chaetopterus pergamentaceus Cuvier. Journ. of Morph., X, 1895. 
