501 



or in a solution of calcium hydroxide, both of which form compounds 

 with the haematoxylin. 



Mucicarmine gives successful results with the glands of Brunner, 

 if the strong stock solution of Mayer, freshly prepared, be employed. 

 In the writer's hands, the solution after twenty-four to forty-eight 

 hours, cannot be filtered and refuses to stain. The strong mucicarmine 

 solution is employed in exactly the same way as the stronger muchae- 

 matein and the results are similar. 



Muchaematein and mucicarmine prepared and applied as indicated 

 above, will stain deeply the secretion contained in cells from the 

 following sources: mucous cells from the various salivary, buccal, 

 lingual, palatine, tracheal and oesophageal glands ; the gastric epithelial 

 cells ; the cells of the cardiac glands of the stomach ; the pyloric 

 glands cells; the neck chief cells of the gastric glands; goblet cells 

 from all sources; and the cells of the glands of Brunner of the 

 rabbit. They do not stain the secretion in cells from the following 

 sources: demilune cells of the salivary glands; serous salivary gland 

 cells, whether from pure serous glands or mixed glands; the serous 

 portions of the palatine and tracheal glands; the glands of v. Ebner 

 in the tongue; the chief cells of the body of the fundus glands of the 

 stomach; parietal cells of the fundus glands; the pancreatic cells. 



In view of these results in glands of known character, we are 

 justified in concluding that these solutions do stain mucous cells and 

 do not stain zymogenic serous cells? 



The transparent cells of the glands of Brunner of the rabbit, 

 when stained in the way indicated above, appear in material fixed in 

 alcoholic bichromate sublimate filled with minute, deeply stained 

 granules which are often arranged in two masses corresponding to 

 the two secondary clear zones described above, a proximal mass near 

 the nucleus and a distal mass near the lumen. A similar subdivision 

 of the secretion in mucous cells into two masses has been observed 

 by Maximow ('01) in the retrolingual gland of the dog and by 

 Kolossow ('98) in various mucous glands. The probable explanation 

 is that the cell stores up new secretion in the neighborhood of the 

 nucleus, the proximal mass being this new formed secretion, the distal 

 mass, the older secretion. 



The conclusion, based on the structure of the cells, and on their 

 staining in muchaematein and mucicarmine, employed as indicated, 

 that the clear tubules are mucous in character seems unavoidable. 

 Similar results I have obtained in all the mammals examined by me, 

 including Didelphys, Canis, Felis, Lutreola, Procyon, Erinaceus, 



