34 
Tobacco, cured and sweated—Continued. 
MISCELLANEOUS SAMPLES. 
Date. | Sample. Oxidase. | Peroxidase. | Remarks. 
1899. | 
Mar. 4 |-Maryland tobacco, Jewell| None .........2..--.|---.-<2ececes--= | 1898 crop. - 
| Waiter by Sesogab4s5osSacs)|seces ses bSesaededs|bosseescogsecses | Flue cured; 1896 crop. 
Sept. 25 || WaSCOnSiIN. 2o-se 5222-2 ere C0! S22 cc es ton. | sae oes Se aae LOO eROp= 
25 | Lititz; Pa, broadleaf =. 22-5 === Oise seeped teats | Se ese ee ees | 1893 crop. 
25 | Lititz, Pa., Habanaseed..-}..-..- C6 Sa eee racen neces | 1897 crop. 
25 Soe Mass., broad- | Moderate .-.-...-..-. Miuchess sees _ 1898 crop, single-leaf cured. 
eat. 
Nov. 1 | Ohio Zimmer Spanish —..| None .---2--- =. =. 2. - INOnG ire Stalk cured, crop of 1898, 
: from Ohio. 
Ohio Zittle Dutehs.2--.--|-s--= DOs ssektae ese Muchese=s-eee From Ohio. : 
Aug. 18 | Hatfield, Mass., Habana | Much...............|..... do ........| Single-leaf cured and 
seed long wrappers. | sweated in cases at 100° 
| FF. 1898. 
18 | Hatfield, Mass., suckers.| Moderate ...........|..-.- GONsecete ee | Do. 
18 | Hatfield, Mass., top of |.--.. GO) sedeesoscccies Moderate ..... Do. 
suckers. | 
18>} Hatheld)) Mass: ground |-Wittle- {2-2--.c--2.-5|-o55< dOnseaseecs Do. 
| _ leaves. | 
Dec; =| Remedios: Cubasecces-es- INONCRasc coe aee eee Mulchaeeeeeecs Crop of 1899. 
Rardido, Culbassce2- eee ane dome ceerenceeee Slight trace... Do. 
Hiaibigwaeos -cenecascceeees MTACO LES seice eee sees Miurcheeee ce Do. 
PMeLtOnRicCO aoseceeeeeee INONG ieee eee Moderate .-..- Poor grade, 1899. 
IEWAIVAKOARIED socosugubeosellecoae (WO) setae Sketeer lee Gye henaus Best grade. 
We learn from these tables that the flue-curing process is unfavor- 
able for the preservation of the oxidizing enzyms, and that sweated 
tobacco has frequently lost its entire content of oxidase, while the per- 
oxidase is more resistant under the conditions of the sweating process. 
From the absence of oxidase in fermented leaves no conelusion can, of 
course, be drawn as to its amount before the sweating was begun. 
The more intensely the sweating process is carried on, the more of these 
oxydizing enzyms will be destroyed. 
TEST FOR CATALASE IN VARIOUS TOBACCOS. 
With regard to catalase, the third oxidizing enzym of tobacco men- 
tioned above (page 22), some experiments were made on various samples 
of cured and sweated tobacco to determine the amount of oxygen devel- 
oped by it from hydrogen peroxide in from fifteen to forty minutes. 
From the energy of this action inference may be drawn as to the rela- 
tively larger or smaller quantity of the enzym in different samples. 
Tobaccos that had been fire-cured, as the plug tobaccos, contain in 
most cases neither oxidase, peroxidase, nor catalase. Such samples 
examined were bright yellow Virginia, black African, and English olive- 
green leaf. A sample of Burley tobacco showed, however, slight traces 
of peroxidase and catalase. Since the temperature in this curing 
method often rises as high as 82° C. (180° F.) these observations do not 
surprise us. Also a flue-cured sample of yellow tobacco from North 
Carolina did not show traces of the three enzyms. Such tobaccos are 
not intended for cigars and are not submitted to the sweating process, 
hence the absence of the oxidizing enzyms is here not of any conse- 
