20 
been treated with the stronger solutions the leaves rolled up badly, in 
some cases never expanding. It frequently happened that the pri- 
mary sheathing leaf remained unsplit, in which case the later leaves, 
prevented for a time from pushing out, doubled themselves into fan- 
tastic shapes, and finally split the sheath leaf on the side, pushing out 
partly or wholly through the lateral opening forced by the bend in the 
growing stem. 
Two lots of plants, the seed for which had been soaked for two hours, 
were allowed to grow for thirty days, when all were carefully taken up 
and measured, with the results given in the following table: 
Height of two lots of plants one month after planting, seed having been soaked for two 
hours. 
: Under = = Rn Total 
Strength of solution. | 5), | 5-10 cm. | 10-15 cm. | 15-20 cm. | 20-25 em. | 25-30 em. | 30-35 em. plants 
No solution (check)-...-. 2 11 ii 10 70 64 28 192 
OFS speUicenibmesea = ===) 2 6 10 32 41 63 38 192 
il (OOP GSiNih Sos5cnesbe0se 8 10 17 21 49 44 24 173 
2ZIOP COMths 65 ooocuoseenc 15 15 12 17 27 7 4 97 
3 TOO COMbs crioaoEAGeKoee 13 15 12 16 8 2 0 66 
SH Celie oeacuocnodeec ati 7 4 8 1 0 0 31 
IM joer Cems. 556066 csnse 8 7 7 2 0 0 0 24 
Motel etic 59 71-| 69 106 196 180 94 775 
The above figures express the height of all plants grown from 1,400 
seeds planted at two different times and allowed to grow for thirty days 
in the sand of the forcing bed. In the first column are included all 
those seeds which had germinated, but failed, even after so long a time, 
to appear above ground. The height was measured from the surface 
of the ground to the extremity of the longest leaf. In the totals it will 
be seen that the check lots and those treated with the 0.5 per cent 
solution gave the same number of plants, and the average height of 
both was about the same. At the time of measuring the plants but 
little if any difference could be noticed in their aerial parts. The 
third series, those treated with a 1 per cent solution, were nearly 
equal in height to the lots untreated and treated with 0.5 per cent 
solution, and to all appearance were well developed. AIl other plants 
were conspicuously affected by the treatment. One of the most strik- 
ing differences noticed between the plants from the treated and the 
untreated seed was in color, those from the treated seed being a deep 
bluish-green, while those from seeds soaked in solutions of 3 per cent 
or more for over thirty minutes were of a much darker green. This 
difference was in some cases maintained during the entire period of 
growth of the plants. 
Equal weights of the aerial portions of plants, representing the most 
marked differences in color, were placed in alcohol and kept for a con- 
siderable time. The alcoholic solutions of the chlorophyll showed as 
great differences aS were seen in the plants. Whether the copper acts 
