37 



The slime was feebly alkaline on the thirty-fourth and sixty-fourth 

 days Avhichever medium was used. 



White Turnip. 



Slices from the roots of smooth, green-leaved (non glaucous), flat- 

 hottomed, edible, white turnips were prepared in th(^ same way as the 

 potato cylinders. 



The hyacinth organism grew well on this substratum and without an}^ 

 marked retardation. On the third da}', at 21° to 23° C. , tlie growth was 

 very feeble in comparison with that of Ps. caiupestris or Ps. j^^^f^^^oli. 

 On the seventh day, at 20° to 23°, the growth was copious over the whole 

 of the exposed part of the cylinder and the fluid was very cloudy, but 

 there was little or no precipitate. On the twenty-second day growth 

 was copious in the air and also in the upper part of the Avater, i. e., 

 there was a l:>ctter growth than in corresponding tubes of potato. 

 After 54 days there was still a copious growth. 



The surface of the slime was smooth and wet-shining, even in old 

 cultures (54 days). 



After 7 days at room temperatures the color in one tube Avas pale 

 yellow, except the scanty precipitate, which was canary 3'ellow. After 

 22 daj's the same culture was pale yellow. In another tube, on the 

 seventh day, the color was unlike anj^ in Ridgway's book, but approxi- 

 mated his Naples yellow (VI-IS). This slime was plainl}^ 3'ellower 

 than the equal h' copious growth in a corresponding tube of Ps. cam- 

 jyestris. At the end of 25 days the slime in the upper part of the tube 

 against the glass had developed a pale reddish-yellow color, quite in 

 contrast with the color of a corresponding tube of Ps. pJiaseolL There 

 was also the merest trace of this coJoi* in the first cultures on radish. 

 After 50 da3^s at room temperatures the slime in one tube was "dirty 

 yellow," while in another it was "pale yellow," i. e., much paler than 

 in a culture of the same age on coconut. In mass, on Avhite paper, 

 this pale yellow slime Avas between Ridgwa3^'s buff yellow and maize 

 yellow (VI-19 and 21). 



After 54 daA's, at room temperatures, the slime snowed no alkaline 

 reaction, l)ut Avas plainl}^ acid to neutral litnuis paper (onlv one tube 

 tested). This red reaction Avas apparent at once and ])ecame stronger 

 as the paper dried. 



A broAvn stain slowly dcA^eloped in the substratum, being clearly 

 visible onlv after 2 or 3 Aveeks. On the tAventy-second day and the 

 thirty-eighth day the substratum was not browned as nmch as in corre- 

 sponding cultui-es on yellow turnips. On the thirty-eighth day the color 

 in one tul^e approximated Ridgway's russet. In another culture of 

 the same age the ])rown was paler, approximating his taAvny olive. 

 On the forty-ninth day the substratum Avas darker than on the nine- 

 teenth, and was several shades darker than in a corresponding tube of 



