36 



No spores could be found in a culture which had grown in the ice 

 chest for 50 days. 



A culture at room temperatures was feebly alkaline to neutral litmus 

 after 50 days. A culture which had been kept in the ice chest for the 

 same length of time, and consequently was not so far advanced in 

 growth, was distinctl}^ alkaline, i. e., more so than the preceding. 

 After 81 days in the ice chest a culture was strongh^ alkaline to neutral 

 litmus. 



No acid reaction was observed. 



No brown pigment was developed. After 49 days at room temper- 

 atures the substratum was as white as when inoculated. 



No cytohydroh^tic action was observed. After the organism had 

 grown on it for 81 da^^s (ice chest) the substratum was as tough as 

 when first inoculated. 



No cr3^stals were observed and there was no decided smell. 



Ps. stewarti grew in much the same Ava}^ on this substratum, but 

 frequenth^ made less growth. The color of its slime was buff \'ellow, 

 and cr^^stals were formed. 



Radish. 



Slices of small, tender, red-skinned, turnip-rooted radishes were pre- 

 pared in the same waj^ as the potato. 



On this substratum the hyacinth organism made a good growth, as 

 the following record shows: 



Stock 211. — Much water. Inoculated February 19, 5 jd. in., from a beef-broth cul- 

 ture 14 days old, and kept at room temperatures. 



February 22, 3 p. m. A good growth on the surface aboAe the water, pale yellow, 

 wet-shining. Fluid clouded, no precipitate. 



February 26. A wet-shining, pale yellow growth o\er the whole exposed surface. 

 A good growth, but not more copious than that in a corresponding tube of P^\ cam- 

 pestris. A moderate amount of precipitate. This is a yellower germ than Pa. cam- 

 jyestris. It shows so, plainly, on all four media (radish, turnip, carrot, and coconut). 



March 5. A copious growth. No brown pigment. 



March 16. No brown stain. 



April 9. The culture has begun to dry out, but there is still about one-half c. c. of 

 fluid in the bottom of the tube. There is a thin pale-yellow precipitate. The sub- 

 stratum has changed color decidedly. The check tubes are still white, but the sub- 

 stratum in this one is of a color not easily described, i. e., unlike any in Ridgway's 

 color system. It approaches his raw Sienna ( V-2) , and if that color had in it a very 

 slight amount of brown it would closely resemble the color of this substratum. On 

 long standing, therefore, a brownish ])igment appears in tubes of radish. 



A 3"ear later this experiment was repeated, using globose red and 

 oblong white radishes. The results were substantially the same. 

 There was a copious, ver}^ wet-shining, very pale-yellow growth, which 

 never became bright 3^ellow like that on coconut. In each case the 

 substratum finally became brown, ])ut this change took place ver}^ 

 slowly, and the color never became deeper than a pale russet (01 clays). 



