13 



clouded," iind + +. ••very feel)ly clouded,'' shows the date at which 

 cloudino- took phice in these tubes, the temperature being the same, 

 i.e., 18- to22oC.: 



Taijlk I. — Sltoinhi/j effect <jf sodium cJiloride on PseudotnoiKis lujacintld in beef broth. 



Stock. 



Method of 

 inoculation. 



Per 



cent 



of 



NaCl. 











N 



ovember 



- 









No. 



of 



tube. 



(■). 



7. 



8. 



r. 



10. 



11. 



12. 



13. 



14. 



15. 



16. 



17. 



5-29 



Needle 



0.5 



















- 



n 



+ 















1 



5129 



Needle 



0. 5 



















+ + 

















2 



529 



Needle 



0.5 























+ 









■ 







3 



535 



Needle 



1.5 











































+ + 





4 



535 



Needle 



1.5 















s 



































5 



535 



Needle 



1.5 















t 































+ + 



G 



529 



2 mm. loop . . . 



0.5 







b 







+ 



















' 



529 



2 mm. loop 



0.5 















+ 



















s 



529 



2 mm. loop ... 



0.5 







1? 

 







+ 



















9 



535 



2 mm. loop . . . 



1.5 







t 























+ + 











10 



535 



2 mm. loop . . . 



1.5 







# ■ 



























+ + 











11 



535 



2 mm. loop ... 



1.5 































+ + 











12 



There was a distinct slight retardation in stock 529, owing to the fact 

 that no alkali was added to it other than that which was natural!}' in 

 the peptone and perhaps, also, to the 0.5 NaCl which it contained. An 

 examination of the table, however, shows that there Avas a very marked 

 additional retardation in stock 535, Avhich could be attributed onh' to 

 the excess of sodium chloride. The retarding influence once overcome, 

 growth proceeded, e. g., in tube 4 clouding was as twice as heavy on 

 November 17 as on November 16, and in tubes 10, 11, and 12 it was twice 

 as heavy on November 15 as on November 13. The date on which the 

 clouding would have taken place in peptonized beef bouillon free from 

 sodium chloride and neutralized to phenolphthalein by means of caustic 

 soda is indicated in the table by ^ (see page 9). 



