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Eelative nutrient value of carbon compounds 85 



Bouillon and peptone water ^^-ith various sugars, etc 85 



Crude vegetable substances 86 



Sugar gelatin 87 



Sugar agars 87 



Sodium acetate 96 



Xutrient starch jelly with sugars, gtmis, and alcohols 96 



Temperature experiments 98 



Thermal death point 98 



Maximum temperature for growth 102 



Optimum temperature for growth 108 



Minimtmi temperature for growth 108 



Formation of acids 109 



Formation of alkalies 110 



Eosolic acid test 110 



Acid fuchsin test 113 



Litmus - 114 



Reduction experiments 114 



^^lethylene blue - . . 114 



Indigo carmine 115 



Litmus * - 117 



Tests for hydrogen sulphide : 127 



Formation of indol 128 



Tests for nitrites 129 



Peptonized beef broths - 129 



Peptonized I'schinsky ' s solution 129 



titrate bouillon 129 



Ferments 130 



Cytase 130 



Invertase - - 132 



Diastase 133 



Trypsin 134 



Lab ferment - 134 



Oxidizing enzymes 134 



Pigment studies 136 



The yellow color 136 



The brown pigment 140 



Nature of the cell wall 143 



Vitality , - - 144 



Length oi life in culture media 144 



Resistance to dry air 145 



Resistance to sunlight 147 



Resistance to heat - 148 



Resistance to acids - 148 



Resistance to alkali 148 



Growth in presence of calciuin sulphite 148 



Growth over chloroform 148 



Means of distinguishing the four species of Pseudomonas 149 



Remarks on the yellow Pseudomonas group - 152 



Characters in common 152 



Other species belonging to this group 153 



