82 



responding tubes of Ps. hyacinthi and Ps. campestris, and the reduced fluid oxidizes 

 back on contact with the air to a color which is bluer than that in the tubes already 

 mentioned, and which resembles that in a tube of Jones's carrot rot organism {Bacillus 

 carotovorus) , where there has certainly been some growth. July 2, feebly clouded; 

 rolling clouds on shaking. July 5, feebly clouded. July 9, clear or very nearly so; 

 no rim or pellicle. July 16, clear; no reduction; fluid a pure blue; a feeble growth 

 after removal to the air, but no marked retardation. 



(c) Salted 2)eptone vxiter vAth rosolic acid. — July 1, no growth; fluid the same color 

 as when inoculated. July 2; clear. July 5, there seems to be a slight deepening of 

 the color, the clouding is not distinct. July 9, not much change. July 16, as on the 

 9th; fluid slightly pinker than in the corresponding tubes of Ps. hyacinthi and Ps. 

 campestris] not cloudy. 



(d) Uschinsky's sohdion. — July 1, no growth. July 2, clear. July 5, clear. July 

 9, clear. July 16, clear; no growth. 



B. 2>yocyaneus pericarditidis: 



(a) Beef broth. — July 1, a slight growth, which has not increased any of late; the 

 fluid is clear and there is no rim, but there is a small amount of precipitate (10 mm. 

 wide), and a bacterial film in\nsible to the naked eye, but distinct under the lens, 

 and covering about one-sixth of the surface. July 2, moderately cloudy; fluid 

 green fluorescent in the upper one-fourth and bearing a thick white pellicle. July 

 5, very heavy clouding and a marked increase of the fluorescence; an abmidant 

 w^hite precipitate, a thin white rim, and a white pellicle which settles easily on 

 jarring. July 9, very cloudy, but fluorescence not pronounced. July 16, fluid 

 well clouded; very ropy; only slightly fluorescent, feebly browned; precipitate 4 

 mm. deep; no retardation of growth after removal to the air. 



(b) Grajie sugar p)eptone ivater imth methylene blue. — Julvl, no growth or only the 

 merest trace; fluid nearly colorless; it becomes greenish in a few minutes on 

 exposure to the air. July 2, fluid clouded, with a thin pellicle; color half reduced. 

 July 5, fluid well clouded and miiformly blue, if any reduction of color it is imi- 

 form; some of the pellicle has fallen; under the fluid there is a thin white rim 

 1^^ mm. wide. July 9, cloudj^ blue with a thin white rim and pellicle. ' July 16, 

 the pellicle has fallen; the fluid is pure blue; there is no distinct fluorescence or 

 reduction of color. 



(c) Scdted 2^eptone ivater vMh rosolic acid. — July 1," a trace of growth; not cloudy, 

 but with a slight precipitate and a membranaceous pellicle visible only under a lens; 

 no rim. July 2, fluid clouded, pellicle more distinct; the color has turned toward 

 pink. July 5, moderately cloudy; no pellicle, that of July 2 lies on the bottom 

 unbroken; there is a thin rim under the surface of the fluid; the latter is now 

 bright pink; it was originally yellowish rosaceous and the miinoculated tubes are 

 still that color. July 9, much as on 5th. July 16, fluid moderately cloudy, color 

 bright pink red. 



B. amylo varus: 



(a) Beef broth. — July 1, clear; no present growth; there is a slight precipitate, 

 chemical (?); it is much less than in case of B. coli. July 2, clear. July 5, clear. 

 July 9, clear. July 16. clear; no growth, unless possibly when first ])laced in the 

 hydrogen. 



(b) Grape sayo.r jh^Iuu,. ivater luith methylene blue. — July 1, no growth; fluid nearly 

 colorless when removed, but soon changing to a greenish blue, as in case of /*.s-. hyacinthi 

 and Ps. campestris. July 2, clear. July 5, clear. July 9, clear. July 16, no reduc- 

 tion; no clouding; fluid "pureM.ie." 



(c) Salted peptone ivater ivith rosolic acid. — July 1, no rim, pellicle, or clouding. 

 A slight rosy precipitate (2 mm. wide), which is possibly chemical; the color of the 

 fluid is the same as when inoculated. July 2, clear. July 5, no growth; no change 

 in color. July 9, as on the 5th. July 16, as on the 5th. 



