84 



jar and as often extinguished. They went out instantly they were 

 depressed below the level of the top of the jar. The checks behaved 

 well. On the sixth day in each one there Avas a well-developed typical 

 growth of the particular organism used. 



The I'esults obtained in the CO3 and on continuing the cultures in 

 the air are stated below. The temperature during the experiment did 

 not vary much from 22° C. 



Ps. hyacinthi; 



(a) Beef broth. — March 20, no growth. March 21, clear. March 23, no growth. 

 March 27, moderately cloudy with rolling clouds on shaking; a slight precipitate. 

 March 31, well clouded; growth retarded by the CO^. 



(b) Potato. — March 20, no growth. March 21, no growth. March 23, no growth. 

 March 27, a typical growth, wet and distinctly yellow on the lower one-half of the 

 exposed x:)art of the potato; that part out of the water is graying. March 31, the 

 yello^^' slime now covers all of the potato out of the water. 



(c) Potato. — March 20, no growth. March 21, no growth. March 23, no growth. 

 March 27, same appearance as in the preceding; growth retarded by the CO2. iMarch 

 31, like the preceding- 



(d) Coconut. — March 20, no growth. March 21, a thin j^ellow growth now covers 

 about 1 sq. cm. ; growth in the air not retarded by the exposure. March 23, a tliin 

 distinct yellow growth now covers 6 to 7 sq. cm. March 27, 9 sq. cm. of Ijright 

 yellow growth. 



(e) Coconut. — ^March 20, no growth. Marcli 21, a thin, yellow growth now covers 

 about 3 sq. cm. March 23, like the preceding. March 27, like the preceding. 



Ps. campestns: 



(a) Beef broth. — March 20, no growth. March 21, clear. March 23, no growth. 

 March 27, no growth. March 31, no growth; fluid still alkaline; it was now rein- 

 oculated with a small amount of yellow slime from potato culture b, and on April 5 

 was well clouded with a yellow rim and numerous zoogloe?e; exposure to CO2 

 appears to have destroyed the organism. 



(b) Potato. — March 20, no growth. March 21, no visible growth. March 23, a 

 feeble pale-yellow growth now covers part of the potato; growth retarded by the 

 COg. March 27, a copious wet-looking, distinctly yellow slime oii the exposed parts 

 of the potato and in the fluid. 



(c) Potato. — March 20, no growth. March 21, no visible growth. IMarch 23, 

 resembles the preceding^less growth Init more color; growth retarded. IMarch 

 27, like the preceding. 



(d) Coconut. — March 20, no growth. March 21, no visible growth. March 23, a 

 thin yellow growth now covers 5 sq. cm. March 27, a yellow growth now covers 

 nearly all the cylinder out of the water; no distinct retardation. 



(e) Coconut. — March 20, no growth. March 21, no visible growth. March 23, a 

 thin yellow growth covers 3 sq. cm. March 27, like the preceding. 



Ps. steivarti: 



(a) Beef broth. — March 20, no growth. March 21, clear. IMarch 23, no growth. 

 March 27, no growth. March 31, no growth; reinoculated with a small amount 

 of slime from the '^oconut culture d. On April 5 the culture was well clouded and 

 had a good rim; exposure to the CO2 appears to have destroyed the organism. 



(b) Potato. — March 20, no growth. March 21, no visible growth. March 23, a 

 feeble, patchy, buff-yellow growth now covers 6 sq. cm. March 27, a typical buff- 

 yellow growth; marked graying of the potato in the air. 



(c) Potato. — March 20, no growth. March 21, no visible growtli. IMarch 23, a 

 feeble, patchy, buff-yellow growth covers 3 s(i. cm.; neither j)otato has grayed. 

 March 27, like the preceding. 



