1)7 



Tahi.e V. — Behavior of I's. hijucndlii, etc., on nutrient starch jelly v/itli various carbon foods. 



Substance 



Organisms. 



added. 



Ps. hyacinthi. 



Ps. campestris. ': Ps. phaseoli. 



Ps. stewarti. 



Lactose 









No increased growth. 









lating effect. 

 Growth very copi- 

 ous, sirupy and 

 with a magnifi- 

 cent production 

 of the yellow pig- 

 ment. 



Dextrin ... 



A stimulating effect. 

 Bright yellow. 

 Growth several 

 times as abundant 

 as in the check tubes. 

 Not sirupy. 







No increased growth. 









Maltose 



Feeble at first, then 







No stimulating ef- 

 fect. Very fee- 

 ble pale-yellow 

 growth. 





several times as 

 much as on check. 

 Bright yellow. 







Galactose.. 



A stimulating effect. 

 Several times as 

 much growth as in 

 check. Slime very 

 bright yellow. 







Marked stimulating 

 effect. As much 

 growth or nearly 

 as much as on cane 

 sugar. 100 times 

 as much growth 

 as on the glycerine 

 jelly. 









Cane sugar 



Copious bright-yellow 

 growth. Several 

 times as abundant 

 as in check. After 

 128 days the starch 

 immediately under 

 the bacterial layer 

 gave a marked re- 

 action with iodine, 

 blue and purple. 

 Litmus reaction 

 feebly acid. 



Copious, smooth, 

 wet-shining pale- 

 yellow growth. 

 Not much more 

 abundant and 

 not so vellow as 

 finally in the 

 glycerinated jelly. 





Marked stimulating 

 effect. A copious, 

 smooth, wet-shin- 

 ing,' buff-yellow, 

 sirupy growth. 







No stimulating effect. 

 Not more growth 

 than in the check 

 tubes. 







No stimulating ef- 

 fect. 









Glycerin... 



Growth feeble for some 

 weeks as if retarded, 

 color pale. After 128 

 days the whole sur- 

 face of the slant (7 

 sq. cm.) was covered 

 with a dense growth. 

 The color was a uni- 

 form dull vellow, a 

 little brighter than 

 wax yellow. The 

 entire surface was 

 shagreened. The 

 starch was not 

 browned. It had 

 lifted a little from 

 the bottom on which 

 was a small amount 

 of vellow fluid due 

 to the solvent action 

 of the glycerol. 

 Neither slime nor 

 fluid were alkaline, 

 both appeared to be 

 neutral whcji wet, 

 and the litmus was 

 only slightly acid 

 when dry. The 

 slime was not sticky. 

 The starch even im- 

 mediately under the 

 yellow layer reacted 

 at once blue or 

 purple with iodine. 



Retardation of 

 growth, of vellow 

 pigment, and of 

 diastasic action. 

 After 24 days, 

 however, a co- 

 pious, sirupy 

 smooth, wet-shiji- 

 ing, rather bright 

 yellow growth 

 over whole slant, 

 and culture then 

 in marked con- 

 trast to Ps. phas- 

 eoli and Ps. 

 stewarti. 



Retards growth, 

 diasta.sic action, 

 and formation of 

 yellow pigment 

 "(onlv one test). 

 After 24 days not 

 one one- hun- 

 dredth as much 

 growfh as in the 

 check or the lac- 

 tose jellv and no 

 distinct "yellow 

 pigmentation. 



No stimulating 

 effect, and appar- 

 ently a distinct 

 retardation. 



21788— No. 28—01- 



