104 



was some retardation. On the sixth day, when next examined, there was a distinct 

 yellow growth over a lai-ge part of the cylinder. On the eighth day this growth was 

 bright yellow and copious. The development of this culture was normal, and con- 

 tinued for a month or more. The 2 tubes put into the thermostat remained free 

 from bacterial growth. Both were taken out on the thirteenth day and left at room 

 temperatures (19° to 26° C.) for 54 days, but there was never any growth. 



(8) Four well-plugged tubes of resistant glass, containing 10 c. c. of strongly alka- 

 line beef broth (stock 286b), which had evaporated to 8 c. c. by long standing, were 

 each inoculated with a large loop from a beef-broth culture of Ps. hi/acinthi 48 hours 

 old, which had been inoculated copiously from a solid culture and was cloudy from 

 growth. Two of these tubes were kept in the dark at room temperatures of 20° to 

 25° C. The other 2 were put into the thermostat at 33.35° to 35.58° C. (mostly 34.32° 

 to 34.55°) during the first 8 days, and after that at 32.45° to 35.55°. 



Result: The 2 check tubes clouded in 48 hours and developed normally. The other 

 2 tubes remained clear as long as they were kept in the thermostat — 37 days for one 

 and. 13 days for the other. The latter was removed on the thirteenth day and kept 

 at room temperatures for 24 days, but no growth ensued. The germs were dead, 

 however, in each tube considerably in advance of the thirteenth day, for 2 tubes of 

 the same beef broth which were inoculated therefrom on the eighth day, using large 

 loops, and left in the dark at room temperatures, remained entirely free from growth 

 as long as the experiment continued (29 days). 



(9) Two cylinders cut from a yellow turnip and steamed in the usual amount of 

 water were inoculated at the same time, from the same culture, and in the same 

 copious manner as the preceding. One of these was put into the thermostat and the 

 other was kept at room temperatures. 



Result: The check tube showed a distinct yellow growth on the third day. On 

 the fifth day this growth was copious and typical for Fs. iiyacinthi. The tube in the 

 thermostat showed no growth on the fifth day and was then reinoculated with a large 

 loop of yellow slime from the check tube. The tube was then shaken tintil the slime 

 was washed over the cylinder and dissolved in the fluid, and the yellow color iuN-is- 

 ible. The tube was then put back into the thermostat. In 26 hours there was a 

 slight yellow growth on the upper part of the cylinder (temperature 34.45°, falling 

 slowly to 33.35° C. ). Two days later there was, apparently, no increase of growth 

 (temperature 34.53° a. m., 34.15° p. m., 34.32° a. m.), and not one one-hundredth 

 part as much growth as in a tube inoculated at the same time for comparison. On 

 the eighth day (temperatures 34.40° to 35.55° C.) growth was A-ery scanty and the 

 color scarcely visible. The amount of growth at this time was not one three- 

 hundredth as much as in the check tube held at room temperatures. On the twelfth 

 day after this reinoculation growth had increased a little, but was still very feeble 

 and certainly not one one-hundred and fiftieth as much as the same culture Avould 

 have given at room temperatures. During these last 4 days the thermostat was 

 considerably cooler, the temperature of the culture chamber ranging from 32.45° 

 to 34.45° C, and being most of the time below 34°. After 49 days in the thermostat 

 a tube of alkaline beef broth was inoculated very copiously from this tube and left at 

 room temperatures 27 days, but no growth ensued, i. e., the vegetative rods were 

 dead and no spores were present. 



(10) Two cylinders of steamed sugar l>eet were inoculated at the same time, from 

 the same culture, and in the same manner as in the two preceding experiments. 

 One of these tubes was put into the thermostat and the other was held at room tem- 

 peratures. 



Result: On the fifth day there was no visible growth in either tube and both were 

 reinoculated very copiously with the solid slime from a turnip culture 5 days old 

 (the check of series 9). The tube which came from the thermostat was shaken thor- 

 oughly before replacing, so that if there were any subsequent growth it might not be 



