105 



confused with any undissolved slime used in making the inoculation. At the end of 

 20 h(Hirs there was a slight growth on the cylinder in eacth tube. On the third day, 

 in the thermostat (temperatures 34.45° p. m., 34.35° a. m., 33.35° p. m., 34.53° a. m., 

 34.15° p. m., 34.32° a. m.) the germs covered 2 sq. cm. on one side of the cylinder. 

 This growth was plainly yellow but extremely thin. On the fifth day (temperatures 

 34.32° p. m., 35° a. m., 34.40° p. m., 34.85° a. m.) there seemed to be a slight 

 increase in growth. This growth was very thin, distinctly yellow, not smooth, and 

 rather dry, i. e., not wet-shining. In the check tube there was from 10 to 20 times 

 as much growth, but not as nnu;h growth as there should have been, owing to the 

 fact that the check cylinder was rather dr\-. On the eighth day (temperatures 34.85° 

 a. m., 34.55° p. m., 35.55° a. m., 35.45° p. m., 34.83° a. m., 34.65° p. m., 34.95° a. m. ) 

 there was some increase, the growth ])eing distinctly yellow, but too thin to hide 

 minute irregularities of the substratum. The volume of growth at this time was not 

 one-fiftieth that in the check tube. Examined microscopically, this growth con- 

 sisted of zo(^gl(Te;e, short rods and long rods. The short rods were single, in doubles, 

 or in fours; the long rods were slender threads, 10 to 20 or more times the ength of 

 an ordinary rod. These threads were numerous and their segments were not well 

 defined. No involution forms were observed or any bodies suggestive of spores. On 

 the twelfth day (temperatures 34.05° a. ni., 33.35° p. m., 34.45° a. m., 33.35° p. m., 



32.75° a. m., 32.45° p. m., a. m.) the growth was meager, thin, dull yellow, and 



its surface was shagreened. There was no yellow slime in the water, but the germs 

 on the cylinder out of the water appeared as if still growing, although very slowly. 

 After 49 days in the thermostat a large loop of slime from this tube was removed and 

 put into alkaline beef ]>roth. This tube was kept at room temperatures for 27 days, 

 but no growth ensued. 



(11) This experiment was undertaken to see if cultures started at room tempera- 

 tures would not do better when put into the thermostat than those which had been 

 inserted soon after inoculation. For this purpose I selected a tube of alkaline beef 

 broth, which had been kept as a check on series No. 8, and a tube of yellow turnip, 

 kept as a check on series No. 9. The turnip culture was put into the thermostat on 

 the fifth day, at which time there was a copious, yellow, wet-shining, homogeneous- 

 looking growtli covering most of that part of the cylinder out of the water. The tube 

 of beef broth was put in on the eighth day, at which time the fluid was moderately 

 cloudy and had thrown down a little yellow precipitate, but had not yet developed 

 any pellicle, rim of germs, or zoogloese. The temperatures were 34.15° to 35.55° dur- 

 ing the first 9 days (once as low as 33.35°) and then 32.45° to 34.45° C. There was 

 no exact check tube for the turnip, but a transfer was made from it into another tube 

 of the same medium; for comparison with the beef broth the other check tube of 

 series No. 8 was used. 



Result: (a) The beef broth in the thermostat at once fell behind the check tube 

 in growtli. On the fifth day the clouding appeared to be feebler than on the start 

 and the trifling precipitate had increased proportionately to the decrease in clouding, 

 but scarcely more. The check tube was distinctly cloudier. On the ninth day there 

 was no increase of j)recipitate. On the twenty-ninth day there was no pellicle, no rim 

 of germs, no zooglcea?, and not more precipitate than on the fifth day, i. e., there 

 appeared to have been no growth whatever during the whole time of the exposure. 

 On this date the check tube was uniformly clouded, showed a yellow rim, and had 

 thrown down a yellow precipitate 12 mm. broad and 2 mm. deep. On the twenty- 

 ninth day a large loop of fluid was taken from the tube in the thermostat and put 

 into a sterile tube of the same heel broth. This tube was under observation 17 days, 

 in conditions very well suited for growth, but no growth ensued. At the end of 40 

 days in the thermostat this experiment was rej)eated, inoculating copiously into 

 alkaline beef broth diluted with distilled water. The tube was kept at room tem- 

 peratures and watched ff)r 27 days, but no growth ensued, i. e., no spores were pres- 



