107 



inoculated therefrom were set away in the dark at room temperatures of 19° to 25* 

 C. (mostly 21° to 23°). The other two were put into the thermos^tat at 34.55° to 

 35.55° for'the first 4 days and then at 32.45° to 34.45° C. 



Result: The cheek tubes were feebly clouded on the third day. They were first 

 examined at the end of 72 hours, and probably clouding could not have been detected 

 more than 6 or 8 hours earlier. These two cultures passed through a normal devel- 

 opment. The other tubes were left in the thermostat 27 day.^, during all of which 

 time they remained perfectly clear. On the twenty-seventh day l)oth were removed 

 to room temperatures and watched for 6 weeks, but they never clouded. When 

 removed from the thermostat each tube still contained about 6.5 c. c. of fluid. 



The following- inferences respecting i^v. hyacinth J appear to be war- 

 ranted by these experiments: 



(a) The organism will not g-row on any medium at 40^ C, and after 7 

 days exposure to this temperature itw411 not grow at any temperature. 

 Probably a much shorter exposure to 40^ C. would kill it. 



(b) The organism will not grow in unneutralized (acid) beef broth 

 at 36° to 38° C, and conseq^iently it is not likely that it will prove 

 pathogenic to warm-blooded animals. 



(c) The organism will not grow in strongh^ alkaline beef broth at 

 35^ to 36.35° C, and after 6 days' exposure to this temperature it 

 will not grow at an}^ temperature. 



(d) The organism will not grow on sugar-beet cylinders at 35° to 

 36.35° C, and after 13 da}^ exposure to this temperature will not 

 develop at any temperature. 



(e) The organism will not grow in strongly alkaline beef broth at 

 34.15° to 35.58° C, and after 8 days' exposure to this temperature it 

 will not grow at an}" temperature. 



(f ) When inoculated very copiouslv from a 3"oung solid culture, the 

 organism grew scantily on yellow turnip at 33.35° to 34.45° C. 



(g) When inoculated very copioush" from a young solid culture, 

 the organism grew very feebly on sugar beet at 34.15° to 35° C. 



(h) Growth already well under way in strongly alkaline beef broth 

 and on ^^ellow turnip was stopped at 34.15° to 35.55° C. 



(i) In 8 days the organism made no visible growth on steamed carrot 

 at 33.35° to 35.45° C, but all of the germs were not killed. 



(k) In 8 days the organism made no visible growth on yellow turnip 

 at 33.35° to 35.55° C, but all of the germs were not killed. 



(1) In 27 davs the organism made no growth in unneutralized (acid) 

 beef broth at 34.55° to 35.55° C, and all were dead before the twenty- 

 seventh day. 



I^s. stewarti refused to grow at 40° C, in Uschinsk^^'s solution and 

 in strongly alkaline beef broth (0 of Fuller's scale). It grows in tlu* 

 thermostat at 36° to 37° C.y on most media, but not so well as at room 

 temperatures of 24° to 25° C. Ps. caiupestrls did not grow at 40° C, 

 and grew not at all or very feebly at 37° to 38° C. — i. e., about as Ps. 

 hyacinthi grows at 34° to 35° C. 



