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separate vial, and the same mode of procedure was followed with the 
whole inside of the thorax, this giving me the entire bee except the 
head, anterior and middle legs, wings, and chitinous walls of the thorax 
and abdomen. Besides these a number of the bees were kept intact. The 
whole series was submitted to the assistant professor of chemistry of 
the Ohio State University, L. M. Bloomfield, to be tested far arsenic by 
the Marsh method. Mr. Bloomfield found the weight of material sub- 
mitted in each case to be as follows : Posterior legs, with pollen attached, 
0.3498 gram: contents of abdomens and honey sacs, 0.0990 gram; ditto 
thorax, 0.0710 gram. After the usual tests to prove the absence of 
arsenic in the reagents it was found that no arsenic was associated 
with the posterior legs or the pollen with which they were loaded, none 
had been left in the thoracic matter, but the material from the abdomens 
gave unmistakable proof of the presence of arsenic. The entire bodies 
of a number of the bees, taken at the same time from the same tree, 
were then washed with diluted ammonia water, three washings failing 
to give a trace of arsenic, but the bodies, after being thus treated, and 
being boiled in water slightly acidulated, gave distinct traces of the 
poison, thus eliminating any possibility of the poison having been 
introduced into the abdominal matter at the time of dissection and 
from the exterior. May 15 a crab apple tree ( Cratregus) was sprayed 
with a mixture of the same ratio of Paris green as before, but in this 
case only the contents of the abdomens were retained. This matter, to 
the weight of 0.1463 gram, treated as in the preceding, gave unmis- 
takable proof of the presence of arsenic. 
Just at this stage of my investigations, chance, if such a thing there 
be, threw in my way still more conclusive proof. A few days prior to 
my last experiment, probably about May 10, a small apple orchard on 
the experiment farm was sprayed with Bordeaux mixture, to which had 
been added Paris green at the rate of 4 ounces to each 50 gallons of the 
mixture. The bloom had at this time nearly all fallen from the trees 
the exceptions being an occasional belated cluster. Three colonies of 
bees, recently brought on to the premises, were located near by. to all 
appearances in a perfectly healthy condition. A few days after the 
application of the poisoned Bordeaux mixture one colony suddenly 
became extinct and a second greatly reduced in numbers, dead bees 
being abundant about both hives. From these colonies I was able to 
secure dead bees, and both honey from uncapped cells and dead brood 
from the hive that had been so mysteriously depopulated. When tested 
for arsenic by Mr. Bloomfield, precisely as with the other matter, con- 
tents of abdomens of the dead bees to the amount of 0.2334 gram 
revealed the presence of arsenic; 3.7061 grams of honey gave no trace 
of poison, while 1.8481 grams dead brood showed it to be present, and 
the entire bodies of the dead bees, thrice washed in ammonia water, as 
before explained, gave traces of arsenic. In regard to the honey 1 can 
only say that it was from uncapped cells, which might and probably 
