COLD-STORAGE EGGS. 



35 



isms was noticed for both white and yolk. The species isolated 

 here are also of a great variety and fairly numerous, as the following 

 list indicates: .Streptotlirix sp.?, B. vulgatus, Sarcina luteaf, Alter- 

 naria sp. % Sarcina flava, Mic. aquatilis, Cladosporium sp. ?, Mic. aerius, 

 B. mesentericus , Bad. ferrugineum, Sarcina subjlava, and Mic. aureus. 

 From this time on until the final examination — which was made 

 when the eggs had been in storage 1 year, 7 months, and 22 days — 

 they were either sterile or inhabited by a few colonies of the more 

 resistant fungi. The number of eggs examined was too small to 

 permit the drawing of elaborate conclusions. The observations are 

 of interest, however, as indicating a line of future investigations. 



Bacteriological examination of fresh eggs. 



No. of 

 sample. 



Re- 

 ceived 

 for ex- 

 amina- 

 tion. 



Portion of 

 egg. 



Number of 

 colonies 

 per cubic 

 centimeter 

 developing 

 on lactose 



agar at 

 35° to 37°C. 



Number of 

 colonies 

 per cubic 

 centimeter 

 developing 

 on lactose 

 gelatin at 

 12° to 15°C. 



Species of bacteria isolated. 



4067 



4068 



4069 



f 5/24/ 

 \ 1906 



1 5/24/ 

 t 1906 

 I 5/24/ 

 i 1906 

 j 5/24/ 

 \ 1906 

 f 5/24/ 

 \ 1906 

 j 5/21/ 

 \ 1906 

 i 10/17/ 

 \ 1907 



r 10/17/ 



1 1907 

 f 1/16/ 

 \ 1908 

 J 1/16/ 

 \ 1908 



White.... 



Yolk 



White .... 



Yolk 



White 



Yolk 



White 



Yolk. 



White .... 



Yolk 



White .... 



Yolk 



White 



Yolk 



\V hite 



Yolk 



White .... 



Yolk 



White .... 

 Yolk 



1 

 190 





 



1 

 



1 



240 

 

 5 

 

 6 

 1 

 2 

 

 1 

 

 1 

 

 





 3 





 

 1 

 

 1 

 3 

 

 

 

 

 

 1 

 

 

 

 

 

 



Mic. cinnabar eus, Fliigge. 

 Myobacterium avium, Streptotlirix farcinica, 

 Sarcina lutea. 



Mic. cumulatus? 



4070 



4071 



4072 



B.C. 551.. 

 B. C. 552... 

 B. C. 6C9a.. 

 B. C. 670... 



Bact. haematoides. 

 Micrococcus, not classified. 



B. mesentencus. 



Saccharomyces , B. Pammelii. 



Fungus. 



1 fungoid colony. 



1 gray fungus. 



Actinomyces. 



« Gelatin plates incubated at 15° to 20° C. 

 Bacteriological examination of cold-storage eggs. 



No. of 



sample. 



Duration of cold storage. 



Portion of egg. 



Number of 

 colonies per 

 cubic centi- 

 meter develop- 

 ing at room 

 temperatures. 







3 mos 



3 mos 



7.5 mos. . . 





/White 



6, 250 



4193 



\Yolk 



3,370 





/White. 



1,280 



4195 



\Yolk 



3,260 



4282 



Total egg 







4283 





1 



B. C 55.. 





11.8 mos .. 

 11.8 mos. 









550 



Total egg 





B. ('. U67. 





1 yr. 7 mos 



1 yr. 7 mos 



22 days 



22 days 



/White. 



at ice - box 

 temperature. 



\Yolk 



1 colony. 



B. C. Oils 



/White 



\Yolk 





 



