CONTENTS. 



Page. 



General plan of the investigation 9 



Information furnished by cold-storage warehousemen 11 



Inception of the work 25 



I. Cold-storage eggs 27 



Plan of work 27 



Physical characteristics 28 



Chemical analysis 29 



Discussion of data 29 



Conclusions 30 



Bacteriological examination 34 



Technique 34 



Discussion of results 34 



Microscopical examination 36 



Fresh eggs. 36 



Cold-storage eggs 36 



Supplementary work on rosette crystals 37 



II. Quail cold-stored under known conditions 37 



Organoleptic tests 37 



Bacteriological examination 40 



III. Chickens cold -stored under known conditions 44 



Organoleptic tests 44 



Bacteriological examination 51 



IV. Market cold-storage chickens 57 



Introduction 57 



Chemical studies 59 



Resume of the literature on the chemical composition of poultry ... 59 



Selection of material for study 61 



Chemical technique 62 



Preparation of sample 62 



Muscle determinations 63 



Total solids and water 63 



Fat 63 



Ash 63 



Nitrogenous constituents 64 



Fat determinations 66 



Analyses of fresh chickens 67 



Analyses of cold-stored chickens 69 



Bacteriological studies 75 



Technique 75 



Examination of fresh and cold-stored chickens 77 



Histological studies 79 



Technique 79 



Examination of fresh and cold-storage chicken muscle 81 



5 



