MARKET COLD-STORAGE CHICKENS. 



79 



Bacterial content of cold-storage chicken muscle. 



Description of sample. 



Incubation 

 tempera- 

 ture. 



Aerobes and facul- 

 tative organisms 

 per gram. 



Facultative and an- 

 aerobic organ- 

 isms per gram. 



Anaerobes and fac- 

 ultative organisms 

 resistant to 80° C. 

 per gram. 



Inner 

 thigh. 



Breast. 



Ab- 

 domi- 

 nal 

 wall. 



Inner 

 thigh. 



Breast. 



Ab- 

 domi- 

 nal 

 wall. 



Inner 

 thigh. 



Breast. 



Ab- 

 domi- 

 nal 

 wall. 



No. 4— storage 14 



C. 

 f 37.5 

 I 20 

 l0 to -1.67 



1 37. 5 

 \ 20 

 [0 to -1.67 



| 37.5 

 \ 20 

 (0 to -1.67 



f 37.5 

 { 20 

 l0 to -1.67 



f 37.5 

 \ 20 

 l0 to -1.67 



1,000 

 5,564 

 3,374 



202 



606 







106 



498 







254 

 254 

 51 



55 

 

 



714 

 1,501 

 1,608 



1,961 



5,202 

 



38 



126 







229 

 45 

 76 



246 



107 







1,995 

 1,995 

 1,621 



2,166 



3,321 









 

 



150 



150' 







250 



1,824 











303 







160 



106 











127 

 



442 

 

 



71 



714 







767 



3,752 









 50 

 



"~U5 

 



35 

 

 



665 



1,164 







1,399 



1,660 







77 

 

 



253 

 

 





 

 





 

 



106 



53 









 42 

 



55 

 

 









 





 

 













 



73 

 

 



No. 85— storage 14 



No. 79— storage2 years 

 No. 82— storage 4 years 

 No.83— storage4 years 









 38 

 





 

 



77 

 

 



126 

 

 



HISTOLOGICAL STUDIES. 



TECHNIQUE/' 



A histological study was made of the muscle of the breast, using, 

 generally, the Pectoralis major, and in a number of cases the study 

 has been extended also to the muscle of the inner thigh and to the 

 walls of the abdominal cavity. 



The tissue was removed when making the bacteriological exami- 

 nation and fixed in alcohol, Zenker's fluid, and Orth's fluid. It was 

 found, for practical purposes, that the latter reagent was greatly to 

 be preferred, both for rapidity of fixing and the final cutting of 

 thin sections. Both the Zenker and the alcohol caused the fibers to 

 break and pull apart so that it is frequently an impossibility to 

 obtain sections which are trustworthy, especially from chickens 

 which have been in storage for a considerable length of time. 



The small pieces of tissue remained in Orth's fluid for from 18 to 

 24 hours. They were then washed in running water for another 24 

 hours, and run through alcohols of increasing strengths until finally 

 absolute alcohol was reached. From this they were put into anilin 

 oil until clear, which operation requires only a few minutes and 

 must be very carefully watched; then into xylol until all the anilin 

 is extracted; next into a mixture of xylol and a paraffin melting at 

 52° C, which mixture is preferably kept at a temperature of about 

 37° C, and finally into a 52° C. melting point paraffin where, with 

 several changes, 24 hours are needed for a complete infiltration. 



« The histological preparations used in this study were made by E. Q. St. John. 



