Isle of Wight Disease in Hive Bees. 23 
I1V—Observations and Experiments bearing on “Isle of Wight” 
Disease in Hive Bees.! By John Anderson, M.A., B.Sc., Lecturer in 
Bee-keeping, North of Scotland College of Agriculture, and John Rennie, 
D.Sc., F.R.S.E., Lecturer in Parasitology, University of Aberdeen. 
(With Plate.) 
(Read 24th January 1916. MS. received 11th February 1916.) 
CoNTENTS. PAGE 
I. Introduction : : ‘ d : : : 23 
If. The Course of Isle of Wight Disease, as observed in three distinct localities : 
(a) Isle of Lewis . : ; ; - : . 24 
Summary : ‘ : : : : 29 
(b) Craibstone—Spontaneous recovery . ; : 31 
(c) Deeside : : : : ; : 32 
III. Investigations bearing on the relation of Nosema apis to Isle of Wight 
Disease 5 5 : ; : : 4] 
Nosema infection experiments. a. 47 
Conclusions ‘ : ; : : 51 
IV. Observations and Experiments bearing on the Infectivity of Isle of Wight 
Disease as distinguished from Microsporidiosis _. 52 
(a) Artificial Infection . : : ‘ ee: ; 52 
(b) Natural Infection : F : : : : £ 59 
Conclusion regarding Infectivity . : 4 : : : 61 
VY. Explanation of Plate . 61 
IL—INTRODUCTION. 
THESE observations were commenced in 1909 by one of us (J.A.), whose 
journal contains a detailed record of the history of each stock of bees in the 
Island of Lewis. In the autumn of 1913 J.F. paid two visits to the Nicolson 
Apiary at Stornoway, and subsequently arrangements were made, through 
Professor J. Arthur Thomson, for continuing the research in association with 
the Natural History Department of Aberdeen University. 
The work here reported on was carried out with the aid of grants 
from the Development Fund and the University of Aberdeen through 
the Joint Committee on Research in Animal Nutrition of the University 
of Aberdeen and North of Scotland College of Agriculture. In the 
work we have been assisted by Mr John Innes, B.Sc., M.B., who has 
carried out most of the examinations of bees for the presence of Nosema, and 
1 This constitutes a preliminary report, indicating the trend of the results so far 
accomplished, Although the number of experiments is not large, stress is laid upon the 
fact that they are the only ones on record in this country which have been made upon full 
stocks of bees living under natural conditions. Further experiments are in progress, the 
results of which will be recorded in a subsequent report. 
