44 Proceedings of the Royal Physical Society. 
Four samples of dead bees were taken. Two of these were from collections 
of bees which had been infected by feeding with Vosema spores nearly three 
months previously, and the remaining two consisted of bees from Isle of 
Wight diseased stocks. 
(a) Pulp of twenty-six Nosema-infected bees yielded, on examination, 
numerous spores in the first and every succeeding drop of fluid taken 
from the mass. 
(0) Pulp of a second (separate) lot of MNosema-infected bees—about a 
dozen bees. Result as in case of (a). 
(c) Pulp of bees, “crawlers,” from two stocks—different apiaries on 
Deeside—same stocks as were used for infection in second Shawbost 
experiment (p. 54)—about twenty bees. Result; No Nosema spores 
observed after repeated examinations. 
(d¢) Pulp made of about two thousand bees from a stock which had died 
out from Isle of Wight disease at Banchory. The fluid, after 
draining off from the solid residue, was tested extensively (twenty 
separate examinations), and Nosema spores were not found. 
Other similar tests are given below: 
1. Thirty “crawlers” from DES1 stock (p. 57) were picked up on 10th 
September. They were allowed to die in a small cage, and on 14th were 
pulped in a little water in the usual way. In this stock there had been found 
previously an unidentified organism. ‘The first field examined in the first 
small drop contained numerous spores of this organism. Nosema spores were 
not seen. 
2. Sample from Anguston, consisting of nineteen “crawlers,” had their 
abdomens pulped and examined for Vosema spores. Negative result. 
3. On 11th September 1915, visited Anguston and found nineteen of the 
stocks had succumbed to Isle of Wight disease since last visit. Took a 
sample of dead bees from the site of one of the dead stocks. 
On 20th September these bees, sixty in number, were pulped, and 
examined. Nineteen samples were dealt with and no Nosema found. 
4. On 25th September 1915, seven bees which had died in transit from 
Glenhouse, Stornoway, were pulped. Negative result as regards Nosema 
spores. These were “crawlers” from the front of the hive, but we have no 
other record of Isle of Wight disease in connection with this stock. Seven 
live ones which survived the journey were similarly treated. Result: Negative 
as regards Nosema. 
5. Six bees which remained of the lot of Carniolans (No. 7) from 
Stornoway were pulped on 25th September. No Nosema was found. 
oe piers 
