48 Proceedings of the Royal Physical Society. 
as a swarm from the South of England in June 1914. They had been examined 
on 30th June, and again on 13th July, and found negative as regards Nosema 
spores and young stages. There were no other hive bees within a radius of 
54 miles. 
On 19th of August, the stock was visited and examined. It covered nine 
combs, and clustered on the dummy. Not a “crawler” nor dead bee was to 
be seen in front of the hive, although the weather was very favourable for 
this. Ten bees, taken at random, were examined for Nosema, and spores 
were found in four, two of these showing a very heavy infection. 
On 7th September, a second sample consisting of twelve bees was 
examined, again taken at random from within the hive, and five were found 
infected, three of them heavily. The day was fine, and there were no indica- 
tions of Isle of Wight disease. | 
On 10th October, the bees were again visited and samples taken, from 
inside the hive as formerly. It was a fine October day, and the behaviour of 
the bees was normal. 
On 13th October nine of the sample bees were examined. They were 
active, flying readily, and their intestines were normal in appearance. No 
spores of Nosema were found. On the 14th, eight more bees were examined, 
again with negative results. On the 22nd, three bees of another sample were 
taken. These arrived alive and active. On examination they were found to 
have the intestine normal, and to contain no spores of Nosema. 
From the foregoing it appears certain that this stock of bees was definitely 
infected with Vosema, but this infection failed to produce Isle of Wight 
disease. 
The following are illustrations of numerous artificial infections with 
Nosema carried out on small lots of bees confined in cages. 
(b) Laboratory Experiments. 
I. On 14th June 1915.—Nosema spores derived from bees of Stornoway, 
stock No. 7 (Carniolans), were used to infect some bees taken from an 
observation hive at Marischal College, Aberdeen. 
On the 17th, Nosema spores were found in plenty in five bees taken at 
random from the infected lot. These spores were in alimentary canal; no 
intra-cellular forms were seen. Our view is that those seen were the spores 
ingested. : 
19th June 1915.—Numerous spores were found in the colon of one dying 
bee, and a few in the chyle-stomach. Young stages were observed in cells of 
chyle-stomach (J. A.). 
