Isle of Wight Disease in Hive Bees. 49 
21st June—A dead bee from infected lot was examined; spores were 
found in colon, but not in chyle-stomach. Many cells of the chyle-stomach 
showed intra-cellular stages (J. A. and J. R.). 
Ist July 1915.—On 30th June, two bees were found dead. When 
examined on this date a few spores were found in both, and a great many 
young stages were seen, especially in one bee. A stained smear showed 
many meronts within the cells, there being frequently four parasites in one 
cell (J. R. and J. A.). 
This infection experiment, through pressure of other duties, was allowed 
to come to an end about this time. But the dead bees were preserved, and 
in September they were found to contain MVosema spores in great abundance. 
At this latter date, however, several attempts to produce infection by means 
of these spores failed. 
Il. 20th September 1915.—On this date thirty bees from Crazbstone 
No. Al (a stock originally Italian, but now consisting entirely of black bees) 
were confined in a small cage, and fed on spore-infected material mixed with 
honey. The spore material was obtained from the 14th June experiment. 
Five bees were killed and their chyle-stomachs pulped and examined, before 
infection, for Nosema spores. Result—negative (J. A. and J. R.). 
On 21st, the bees were again fed with the spore-laden material. Up 
to 25th the behaviour of the bees was normal; they fed freely, and 
were quite active. There had been no mortality except in the case of 
three, which died on the 21st. In one of these ingested spores were found. 
On 26th, one bee died and one was killed. No spores were found in 
either of these. 
On 28th September, two died and two were in a weakly state. No spores 
were found in any of these.. 
On 29th, one died and no spores were found. The bees were fed on 
Scholtz candy. 
The intestine in the last examined bee was whitish, and not unlike the 
appearance of a Nosema infection, but, as stated, spores were not observed. 
On 7th October, fourteen bees which had died were pulped. These were 
black bees: no Nosema spores were found. 
It is clear from the foregoing that the spores used in this experiment 
failed to infect. | 
III. On 30th September, a small lot of Welsh bees were caged and fed on 
Nosema-infected material of 15th June. Spores were present in the material 
fed. A similar number of bees were caged as a control, and fed on clean 
candy. Feeding with the infected material, in the case of the first lot, was 
continued on the Ist and 2nd October, after which clean candy was given. 
VOL, XX. D 
