50 Proceedings of the Royal Physical Society. 
On 7th October, it was found that sixteen bees of the infected lot had died. 
These were pulped (abdomens) and examined for Nosema spores. Free spores 
in moderate numbers were found ; two or three in every field examined of 
the first drop taken. One live bee examined gave a negative result. In the 
control, two bees had died. These were pulped (abdomens only), but no es 
were found. ; 
16th October 1915.—Five bees dead. These were pulped on 18th, and 
Nosema spores were found. Other three were examined, and spores found in 
all. Other six were pulped, and spores were again found. All the control - 
bees had died at this date. 
From these results it seems probable that no infection had taken place. 
IV. 4th January 1916.—On this date all the Desswood stocks were in 
flight. The opportunity was taken to procure a number for experiment, and 
samples were captured on the alighting board of the “Kelly” hive. These 
were fed with candy, which on the same day was well smeared with pulped 
matter from bees of the June experiments. This matter was found on 
preliminary tests to contain Nosema spores in plenty. 
The bees were kept in a small wooden cage with glass cover. By the 6th 
several had died. Nine were removed at this date, but of these, two were 
dead shortly after caging, having probably been hutt. 
6th January 1916.—Two were allowed out of the cage, and these flew 
quite strongly, and one of them defecated. 
Sth January 1916.—Other six bees were found dead. One released 
proved unable to fly, although it was quite active. When lifted it used its 
sting. This bee had chyle-stomach, pale in colour, and colon dilated with a 
pasty mass of pollen. Search in the dead bee and in the surviving one failed 
to find any trace of Nosema spores. Another live bee very active was taken, 
killed and examined. This bee had a pale chyle-stomach and inflated watery 
colon. Numerous free spores were found in the latter, but a very thorough 
search failed to discover any trace of infection in the chyle-stomach. 
12th Januarg 1916.—On the 12th all the bees had died. These 
(thirteen) were pulped, and examination showed free MVosema spores in 
moderate numbers. | 
V. 24th June.—Infected two boxes of “Kelly” bees with WVosema 
material from No. 7 Stornoway hive. 
Ist July Examined two bees which had died. Planonts seen in both 
bees and intra-cellular forms in stained film. No free spores seen. 
Sth July.—On this date the chyle-stomach of a bee of this experiment, 
taken alive, was found on examination to contain enormous numbers of 
spores and planonts. Spore clusters common. 
