56 Proceedings of the Royal Physical Society. 
not occur in other two similar experiments, it is not clear that this was due 
to the artificial infection. There were specially adverse circumstances in 
the present case. In the other two instances of attempted infection, the bees 
were maintained in good general external conditions. It should be noted 
further that Nosema was not found at any time during the experiment. 
The foregoing experiments in artificial infection were made in Lewis upon 
isolated stocks. The following were carried out in Aberdeen. 
No. 5.60 DD. May 1916, 
This stock, which stood in an apiary in a district suffering badly 
from Isle of Wight disease, was found, on 24th September 1914, to have 
large numbers of bees on the alighting board, and groups of “crawlers” 
on the grass around. The weather conditions were fine and sunny. The 
same thing was observed on the following day, and the hive and bees were 
removed to an observation area in Aberdeen. For a few days after this the 
stock seemed to be doing well enough and a good deal of pollen was being 
taken in. On fine sunny days, however, “crawling” became very evident, 
and on some occasions it was very extensive. As recorded elsewhere a 
sample of the “crawling” bees was utilised in an infection experiment 
(p. 53). 
On 7th October, which was a fairly good day with a little sunshine, 
“crawling” was again in evidence, but pollen was still being gathered. 
About this time it was reported that three other stocks from the same apiary 
were suffering from the disease. All these eventually perished (p. 34). 
Up to 17th October, bees ‘‘crawled” every fine day, but the last of them 
perished in the early winter, leaving abundance of stores in the hive. 
On 19th May 1915, a fresh stock arrived from the South of England, 
Italian hybrids, fully covering ten combs crowded with brood. The queen 
had apparently been recently lost, but there were numerous queen cells on 
the combs. The new stock was put in the hive in which the above-mentioned 
stock, 60 DD, had been housed. There was no disinfection of any kind, but 
the dead bees and the old combs were removed. The honey was drained 
from some of those old combs and fed to the new stock. 
On 5rd June, a swarm came off and was duly hived in a perfectly new 
hive on frames with full sheets of foundation. This swarm was also fed on 
honey drained from the old combs left by stock 60 DD. 
On the afternoon of the following day a second swarm came off and was 
hived in a straw skep. This second swarm received none of the honey from 
the old combs, but was fed on a little honey bought in the open market. 
