Note on Trypanophis grobbeni. 63 
The nucleus stains bluish in hemalum and eosin — grey in iron- 
hematoxylin. The position of the nucleus is constant, its shape more or less 
so. One side is always formed by the side of the body opposite to the 
undulating membrane. Its inner boundary is not very sharply defined from 
the coarser cytoplasm. 
Keysselitz has figured a substantial kinetonucleus from which the two 
flagella spring directly. No such body is visible in my preparations. The 
free flagellum springs from a more or less distinct basal granule, in the 
position shown in the figures. Close to this granule, in most of the figures, 
can be see a deeply stained body, to which may perhaps be applied the 
name of kinetonucleus. This body, when distinguishable, is surrounded 
by a halo of clear cytoplasm. It must be confessed that in many cases 
nothing so definite as the above can be made out owing to the 
confusion of a number of small darkly stained granules in this region— 
as ég., in Plate II. fig, 3.1 
The attached flagellum originates from the edge of the body at a point 
either on a level with the basal granule of the free flagellum or a little in 
advance of it. 
The base of the narrow undulating membrane is sometimes marked, as in 
Keysselitz’s figures, by a distinct line throughout its length (see Figs. 4 and 6). 
Especially may this be the case in hemalum and eosin preparations in 
which the anterior end of this basal line may be quite intensely stained, 
Just before the basal line joins the flagellum there is a widening of the 
space between them, and, in consequence, the basal line dips inwards, The 
point of union may be marked, as in Figs. 1 and 3, by a darkly stained 
thickening or granule, but in Fig. 2 and in the hemalum preparations this 
cannot be seen. 
The “chromatic bodies” are embedded in a row; their arrangement and 
relation to the cytoplasm are best shown in Fig. 1. The foremost of them 
may be just behind the “kinetonucleus,” when visible, or there may be one 
or two in advance of it as in Figs. 1 and 2. | 
They take up eosin very readily and stain intensely in iron-hematoxylin. 
Keysselitz figures a second partial row of smaller “chromatic 
bodies” in the anterior half of the body. The only trace of this I 
have been able to find is a tendency among the more anterior bodies 
of some specimens to duplicity—partial as in Fig. 4 or complete 
as in Fig, 5. 
As to the clear zone about the kinetonucleus, a similar condition of 
1 Figs. 1-6, x approximately 1600 ; Fig. 7, x 1800. 
VOL, XxX, E 
