Ameeba proteus for Class Purposes. 181 
culture (Journ. Hxp. Zool., vol. xxiv., No. 1), by means of wheat, fell into 
my hands. By adding wheat grains to the mud containing Amabe, and 
by having sufficient water-weed, I was able to keep stocks alive throughout 
the winter of 1917-1918, the weed dispensing with the necessity of 
oxygenating the water by artificial means. In some cases the Amabe 
“Jost hold” in the culture, ciliates or crustaceans or oligochete worms, 
etc., making headway, to the almost total extinction of the first. In 
practice, therefore, it was found advisable to pick out the Amebe from 
the mud to a great extent, and thus give them a good start in the new 
culture. By dividing up into two lots a successful culture, and then adding 
water and new food-supplies to each sub-lot constantly during the warm 
sunny months of 1918, I have at the moment of finishing this note, 
8th September 1918, millions of Amebe in laboratory cultures. 
The tributary of the Allander was examined at intervals during the 
winter and spring of 1918. From December to May the Amwbe were 
found to disappear, this disappearance coinciding roughly with the period 
of encystment described by Miss L. Carter In June they began to re- 
appear, at first small in size and few in numbers, and up to the present 
the yield is not so abundant as in 1917. This may be due to the fact 
that the water has receded somewhat from the original shores of the 
“pond,” the early summer having been a dry one. Laboratory cultures 
of this same material, possibly because kept at a uniform temperature with 
a constant food-supply, multiplied at a uniform rate. 
Having thus determined the necessary conditions for the successful 
capture of Amewbw in the open, and for their culture in large quantities 
under artificial conditions (ze. a plentiful oxygenation with a liberal food- 
supply), it was found interesting, during the summer of 1918, to apply these 
principles to the detection of other sources of supply. The results of these 
investigations were satisfactory, and a list of the places so far investigated 
is given below. Further investigation of similar “sites”? would reveal very 
many more sources. It must be remembered, however, that all natural 
“sites” are’ subject to fluctuations. 
1, Tributary of the Allander draining Dougalston Loch, just before it 
enters the Dougalston Estate. This tributary is connected up with Bardowie 
Loch, the source of much organic material. The tributary itself brings down 
much débris which is mostly deposited at this place. The water of the 
tributary abundantly oxygenates the mud. 
2. Overflow from the last loch on the east side of the old Strathblane 
11915, Carter, L. A., ““The Cyst of Ameéda proteus,” Proc. Roy. Phys. Soc., Edin., 
yol, xix., No, 8, p. 204. 
