18 
discreti, 190-250 u diam., vel elliptici, 250-280 u 160 u. Asci ovati, BOu x 26-33 u, 
vel clavati, 66-70 » x 22-23 u, ad apicem incrassati, octospori, paraphysati; paraphysibus SS 
filiformis, mox mucosis praesentibus. Sporae distichae v. tristichae, oblongae, utringue = 
rotundatae, hyalinae (an semper?) medio v. paullo supra medium septatae, deinde trans- ' 
verse 5, rare 7 septatae, septis tenuissimis, cellulis pluribus v. omnibus septo longitudinali 
divisis, plasmate granuloso, 22—24 u x 9-10 u. 
Hab. in foliis Halleriae lucidae, Louis Trichardt, 8.4.19, leg. Putterill [11847]. 
18. Diplochorella amphimelaena (Mont.) Theiss. and Syd. 
Aan. Myc., 1914, p. 277. 
Syn. Dothidea amphimelaena Mont. 
Ann. Sc., Nat. II, Ser. t. XX, p. 372. Syll. Crypt., p. 222. 
Homostegia amphimelaena Sacc. Syll. Fing. II, p. 650. 
Phyllachora osyridis Cke., Grevillea XIII, p. 64. 
Dothidella osyridis Berl. et Vogl., Syll. Fung. IX, p. 1038. 
Dothidella osyridis var. tassiana Sacc., Syll. Fung. XIV, p. 676. 
Microcyclus osyridis Sace., Ann. Mye. II, 1904, p. 165. 
Microcyclus tassianus Syd., Ann. Myce. II, 1904, p. 165. 
On Osyris compressa, Muizenberg, C.P., 1883, MacOwan (Rabh. Wint. Fung. Eur. : 
3562) [3902]; Port Elizabeth, 23.3.11, Doidge [1243]; St. James, Capetown, 10.12.11, 
Pole Evans [1982]; Kloof Neck, Capetown, 24.2.12, Doidge [2155]; Muizenberg, 12.4.12, 
Beardmore [2292]; Belmont Valley, Grahamstown, 15.11.17, Doidge [10954]; Kalk Bay, 
January, 1918, Potts [11302]. 
Compound stromata, circular, 2-3 mm. diam., forming flat, raised discs on both sides 
of the leaf, with almost vertical edges, silver grey on the surface, thickly beset with 
erumpent punctiform, dull black bodies about 50% diam., which eventually cover the 
whole surface. The densely crowded loculi develop under the epidermis—the apex of 
each separately rupturing the epidermis—and become somewhat raised above it. Gradually 
the part of the epidermis intermediate between the apices of the loculi disappears and 
the surface becomes altogether black. The leaf is normally 500-550 u thick, but through 
the action of the fungus on both leaf surfaces it is often up to 1300 u thick. The central 
leaf tissue is colourless and free from fungous stroma to a thickness of 700 1; above and 
below this the stromatic layer develops, which is again differentiated into two layers, the 
outer composed of the loculi and the inner of the basal hyphal stroma. The first consists 
of closely crowded, perithecium-like loculi, which are 120 4 high and 85-95 u diam., and 
which are more or less connected laterally. From the base of the loculi the hyphae of the 
vegetative stroma run vertically inwards, forming a fairly sharply defined stromatic plate 
120-170 » thick. The loculi are typically composed of grey-brown, laterally compressed 
cells which are 14-15 u long, 5 u thick, and 8-9 » broad, and are in close contact by their 
flat sides. These hyphae sometimes penetrate inwards in more or less compact strands, 
but only to a limited depth, and the intermediate leaf tissue is not destroyed. Towards 
the base of the loculi the greater part of the flat cells become polyhedral or regularly elliptic 
and somewhat smaller and form a sort of wall round the loculi consisting of a few concentric 
layers of cells; they also fill up the spaces between the loculi; the cells are dark brown 
and thick walled. There is apparently no stromatic connection between the stromata on 
the upper and lower leaf surfaces. 
Asci aparaphysate, basal, short cylindrical, 35 u x 12 u, apedicellate, eight-spored. 
Spores polystichous, hy aline, two-celled, 12-13 u x 4 u, constricted at the septum ; lower 
cell twice the length of the upper. 
