New Species of Plethodon 19 



polymorphic locus in Table 2. Thus the D values between P. aureolus and 

 the other two species indicated in Figure 4 are slightly higher than they 

 would be if this Pep difference had not been detected (see Fig. 3.) 



Although there is little or no evidence for hybridization between P. 

 teyahalee and P. glutinosus in the samples from locality 4, at locality 44 

 there are 4 individuals in a sample of 17 that have dorsal spots of interme- 

 diate coloration. In addition, there is a low frequency of P. glutinosus 

 electromorphs at all of the loci that differentiate the two species (Alb, Est, 

 Ldh (muscle), Pt-2, and Trf). This is interpreted as evidence for hybridiza- 

 tion between the two species at this locality. It is not surprising that P. 

 teyahalee and P. glutinosus hybridize in some areas and not in others, since 

 this same pattern occurs between P. teyahalee and P. jordani in the 

 southern Appalachian Mountains. 



ACKNOWLEDGMENTS.— I wish to thank the many persons who 

 aided in the field and laboratory work, especially Ivette Aguirre, Debra 

 Glover, Karol Jackson, Scrap Oktay, Robert Peabody, Joshua Schwartz, 

 Jeffrey Streicher and Susan Fogle. Richard L. Hoffman suggested the 

 scientific name. I am also grateful for the financial support of the work by 

 the National Science Foundation (grants DEB-79-03858 and DEB-81- 

 17973) and the Computer Science Center of the University of Maryland. 

 David B. Wake made helpful comments on the manuscript. 



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