78 



Christopher King Beachy 



lowest degree of variability, as expected. But A. maculatum did not 

 exhibit the highest degree of intraclutch variation as was expected 

 (Fig. 2). 



Interclutch Variation 



Because embryo diameter begins to increase in late developmental 

 stages, only those clutches of D. santeetlah, D. ochrophaeus, and E. 

 wilderae collected earlier than Harrison developmental stage 30 were 

 used in this analysis. All egg size data for A. maculatum and D. 

 aeneus were analyzed. Mean egg size was determined for each clutch, 

 and those data were used to calculate a CV of egg size for each 

 species (Table 1). In this analysis, A. maculatum did show the greatest 

 variation in egg size (Fig. 3). Except for E. wilderae, all species 

 CVs were significantly lower than that for A. maculatum. D. aeneus 

 was predicted to exhibit the lowest degree of variation in egg size; 

 only D. ochrophaeus has a lower CV, although this difference was 

 not significant. 



A. maculatum 



E. wilderae 



D. santeetlah 



D. aeneus 



D. ochrophaeus 



6 8 



Pooled CV 



10 



12 



14 



Fig. 3. Coefficients of variation (CV) of pooled (interclutch) variation in 

 egg size for five species of salamanders. The species CVs are tested with 

 an F-test of the squared CVs (Lewontin 1966). Vertical lines indicate means 

 not distinguishable by pairwise F-tests (P > 0.05). 



