84 Stephen J. Walsh and Brooks M. Burr 



METHODS AND MATERIALS 



Over 220 adults, from the institutions listed in Acknowledgments, 

 were used for morphological comparisons between major drainages or 

 ichthyofaunal blocks as designated by Burr (1980). All measurements 

 were made to the nearest 0.01 mm with dial calipers. Terminology of 

 dentition follows Hubbs and Potter (1971) and Potter (1980). The oral 

 discs of some specimens were lightly stained with alizarin red S to facili- 

 tate tooth counts. Methods of counting and measuring were those of 

 Vladykov and Follett (1958, 1965). 



The life history of L. aepyptera was studied at Terrapin Creek, 

 Graves County, Kentucky, near the Tennessee border. Collections were 

 made from the state line north to 0.8 km south of Bell City, Kentucky. 

 Intermittent sampling from 23 March 1978 to 4 June 1980 resulted in 

 138 specimens, which were fixed in 10% formalin and later transferred 

 to 70% ethanol. Frequencyof occurrence versus lengths of ammocoetes 

 taken at Terrapin Creek on 4 June 1980 were plotted and the curve 

 smoothed using 7 mm sliding averages (Hardisty 1961; Hardisty and 

 Potter 1971; Rohde et al. 1976; Taylor 1965). Lampreys were blotted 

 dry and weighed to the nearest 0.1 mg on a Sartorius analytical bal- 

 ance. Egg diameters were measured with an ocular micrometer at a 

 magnification of 25-50X; no correction was made for shrinkage. 



Collections of L. aepyptera examined, all from Kentucky, are listed 

 below by major drainage, county, catalog number, locality, and date. 

 The number of specimens is shown in parentheses. 



Big Sandy River Drainage.— Martin Co.: UL 7945 (1) Rockhouse Fork of 

 Rockcastle Cr. at Stidham, 27 March 1956. Floyd Co.: UL 5946 (3) John's 

 Cr. below Dewey Dam, 27 March 1956. Morgan Co.: SIUC uncat. (4) 

 Open Fork of Paint Cr. at ford, 24 March 1973. 



Licking River Drainage.— Montgomery Co.: INHS 78829 (2) creek, 1.6 km W 

 Means, 12 April 1968. Rowan Co.: UL 5228 (16) Licking R. at Cranston, 

 12 April 1941. Menifee Co.: UL 4983 (1) Beaver Cr., 8 April 1954. 



Kentucky River Drainage. — Wolfe Co.: KNPC uncat. (2) Swift Camp Cr. just 

 upstream from confluence Red R., 3 May 1980; UL 1014 (1) Red R. near 

 Hazel Green, 24 November 1960. 



Upper Cumberland River Drainage.— McCreary Co.: WCS 720-01 (9) Taylor 

 Branch, 8.8 km E Whitley City, 16 April 1977. Whitley Co.: INHS 79259 

 (1) Youngs Cr., 1.6 km W Clio, 19 March 1978. Rockcastle Co.: UL 2015 

 (1) Clear Cr., 3.2 km S Disputanta, 3 April 1980; EKU 168 (1) Clear Cr., 

 3.2 km SE Disputanta, 20 March 1966. 



Salt River Drainage.— Hardin Co.: KNPC uncat. (3) Clear Cr. downstream 

 from overpass E Colesburg, 24 March 1978. Bullitt Co.: USNM 164102 

 (15) Knob Cr., 28 March 1950; UL 4791 (20) Knob Cr. near Mitchell Hill 

 Road, 25 March 1950; UL 4794 (73) Knob Cr. 9.6 km NW Shepherdsville, 

 25 March 1950; INHS 78228 (1) creek, 9.6 km S Fairdale, 7 April 1968. 



