TESTS WITH DDVP APPLIED TO THE FLOOR OF AISLES ONLY 
Procedure 
In the third series of tests, DDVP was dissolved in tetrachloroethylene 
and sprinkled on the floor of the aisles in 3 type C (closed) warehouses. 
DDVP in 3 gallons of solvent was applied in each of the 3 warehouses in 
amounts that resulted in application rates of 1, 2, and 10 grams per 1,000 
cubic feet of total warehouse space, respectively. The warehouse temperatures 
averaged 72°F, A wind of 10 to 15 miles per hour persisted for the first 8 
hours of the tests. 
Two and one-half hours after application of the DDVP, sets of 8 cages, 
each containing 25 adult beetles and a l-inch section of midrib bearing eggs, 
were placed in warehouses treated with l- and 2-gram rates. Sets were re- 
moved after 1, 2, and hours! exposure. Other sets were placed in the ware- 
houses 22 hours after treatment and exposed ); hours, and others were placed 
28 hours after treatment and exposed 20 hours, 
Before application of DDVP at the l0-gram rate, 16 test spikes were in- 
serted in hogsheads, 8 placing larvae at depths of 1, 3, and 5 inches, and 8 
placing adults at the same depths. One-half of each lot was exposed 2); hours, 
the other half 8 hours. 
Six white rats of the Sherman strain were placed in each warehouse be- 
fore treatment and left undisturbed for 7 days. Two mature rhesus monkeys 
were placed in warehouses which had received applications of 2 and 10 grams 
per 1,000 cubic feet. Four human volunteers were exposed to DDVP vapors for 
the equivalent of 2 working days. On the day after application, 2 of the men 
spent 8 hours in the warehouse treated at the l-gram rate, and 2 spent 8 hours 
in the warehouse treated at the 2-gram rate. The next day all ) men entered 
the warehouse treated at the l0-gram rate. One stayed ) hours and 3 stayed 
7 hours. Blood samples were taken from test animals and the volunteers be- 
fore and during the exposure periods. 
Air samples were collected at various intervals from the treated ware- 
houses for analysis of DDVP content. Samples were collected on Celite 
absorption columns and were analyzed by the total phosphorus method (3). 
Results 
The insect mortality results are given in table 3. The increased 
lengths of exposure gave progressive increases in mortality of adult insects 
exposed in the free air spaces, but only one of the dosage exposure combina- 
tions caused mortality of all those insects, The 10=gram application rate 
did not inhibit egg incubation. There was no significant mortality of either 
adults or larvae at any depth in the tobacco hogsheads with exposures up to 
48 hours and an application rate of 10 grams. 
aeons 
